| Objective:A BALB/c mouse model consistent with Atopic dermatitis AD was established,the expression of S100A11 protein in the lesion of the mouse model was analyzed,the levels of IL-6,IL-4 and IgE in the serum of the mouse model were also detected.To investigate the expression of S100A11 protein and the changes of serum IL6,IL-4 and IgE in the skin barrier dysfunction of atopic dermatitis.Methods:A total of 40 female BALB/c mice with an SPF grade of 20 ± 2 g were selected,Week age is 6-8 weeks.Were randomly divided into two groups(AD group and normal group),Each of the 20.The mice were dorsified on the back of the mice 7days before the start of the experiment,8%sodium sulfide starch solution for the hair removal agent,hair removal area of about 8cm2.AD group modeling method is:On the first day of the experiment,100μl of 0.5%DNFB sensitizer was applied to the dorsal hair removal of mice,2-6 days without any treatment,7-35days during the week to apply sensitizer 2 times,Each time 70μl,a total of 9 times.Normal group smear no DNFB acetone/olive oil solution,each dose of time,with the AD group.After modeling,the mice were anesthetized with ether,The mice in the AD group and the normal control group were removed to remove the right eye blood,After standing at room temperature for 30 min,the supernatant was centrifuged and the concentration of IL-6,IL-4 and IgE in the serum.The tissues of the mice were stained with HE and the protein content of S100A11 was determined.Consequence:1.Mice skin inflammation score score:AD group skin inflammation score(8.00)higher than the control group(0.00),the difference was statistically significant(P<0.05).2.Pathological HE staining results:normal group of normal mice,no inflammatory cell infiltration.AD group of mice sensitized skin can be seen epidermal shedding,necrosis,part of the dermis can be seen increased fibrous tissue,showing a large number of inflammatory cell infiltration.3.Spleen index statistics:AD group mice spleen index(2.08± 0.22),normal group(1.72± 0.13),AD group was significantly higher than the normal group,the difference was statistically significant(P<0.05).4.Scratch count statistics:AD group of mice scratching the number of times(14.29 ± 1.77),normal group(1.19 ±0.92),AD group was significantly higher than the normal group,the difference was statistically significant(P<0.05).5.ELISA test results:AD group serum IL-4(97.64 ± 4.59)pg/ml,normal group(64.80 ± 3,23)pg/ml;AD group serum IL-4(84.66 ± 3.85)pg/ml,normal group(49.32 ± 3.12),AD group serum IgE(17.63 ±4.11)μg/ml,normal group(10.40±2.39)μg/ml.The above results were significantly higher in the AD group than those in the normal group(P<0.01)The correlation coefficient between IL-6 and AD was 0.588,which was positively correlated(P<0.01).6.Western Blot test results:The relative expression of S100A11 in AD group was lower than that in normal control group,the difference was statistically significant(P<0.05).7.The relative expression of S100A11 and the correlation coefficients of serum total IL-4 and IL-6 were-0.667 and-0.809,respectively(P<0.01).Conclusion:1.It is feasible to establish AD mouse model with DNFB.2.The levels of serum IgE,IL-6 and IL-4 in AD group were significantly higher than those in normal group,IL-6 was positively correlated with inflammation degree,Suggesting that inflammatory response to DNFB-induced pathogenesis of AD in mice,IL-6 may become a new indicator of the degree of AD inflammation.3.The levels of IL-4 and IL-6 in serum of AD model group were significantly decreased at S100A11,And S100A11 and IL-4,IL-6 have a negative correlation,suggesting that S100A11 deficiency may be one of the initiating factors of inflammation in the pathogenesis of AD. |
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