Effects Of TiO₂ Film With High Invasive Structure On The Titanium Implant Trabecular Structures:A Micro-CT Evaluation

Objective: In this study,two kinds of surface treatment of implant in rabbit femoral condyle to establish animal models,features around the trabecular bone was evaluated using Micro-CT planting,to explore the influence of different implant surfaces on peri implant bone trabecular structure.Materials and methods: 18 healthy New Zealand rabbits were under general anesthesia in rabbit femoral condyle after implantation with micro arc oxidation treatment of high invasive TiO₂ films and SLA implant.According to the principle of random,random side as the control group（SLA group）,the other side as the experimental group（MAO）,making a record of the rabbits.After 4 weeks and 8 weeks,the rabbits were killed,taken specimen to make the Micro-CT scan and 3D reconstruction.It is a total of 36 specimens of bone scan,experimental group 18,control group 18.Then we use CTan software to select interested area to analyze the experimental group and the control group of peri implant bone morphological parameters interested area of trabecular bone,including bone volume fraction（BV:TV%）,trabecular bone volume（Tb.N）,trabecular separation（Tb.Sp）,trabecular thickness（Tb.Th）,trabecular structure model index（SMI）and connectivity density（Conn.Dn）.Using SPSS20.0 statistical software for statistical analysis,independent samples t test and paired samples t test,to set P<0.05 for the difference was statistically significant.1.Gross observation:After the completion of the operation,the rabbits of good state,daily diet,excretion and activity is good,no incision wound dehiscence,no swelling pus.Checking the surgical site of rabbits,no implant loosening and falling.Rabbits were without claudication.2.In vitro specimens:After 4 weeks and 8 weeks,the animals were sacrificed,the skin cut,the soft tissue separated,the femoral cut.The surface of the implant was covered with new bone.3.Micro-CT reconstruction imageIn the three dimensional images reconstructed from Micro-CT,two kinds of surface treatments were formed trabecular bone structure around the implant in the fourth weeks.In group MAO,it was found that trabecular bone was dense in the group,and there was a significant osseointegration.The trabecular bone was small and sparse in the SLA group,and osseointegration was not as obvious as that in the MAO group.In the eighth weeks group,two treated implants could be seen the formation of trabecular structure around implant.Bone trabecular structure is the thick and dense.Osseointegration was more obvious than 4 weeks group.Bone trabecular structure of MAO group is more dense than SLA group.Trabecular bone spacing is small,closely connected,obvious osseointegration than SLA group.4.Micro-CT trabecular bone parameters analysisOver time,BV:TV,Tb.N,Tb.Th and Conn.Dn values in the 8 week group increased,Tb.Sp and SMI values decreased.BV:TV,Tb.N,Tb.Th and Conn.Dn values of MAO group decreased in the 4 week group compared to the SLA group,and the Tb.Sp and SMI values decreased in the group.At fourth weeks,significant difference in MAO between SLA group and the SMI value（P<0.05）.At eighth weeks,Tb.N,Tb.Sp,significant difference in the MAO between SLA group and the Conn.Dn value（P<0.05）,BV:TV value at fourth and eighth weeks,the difference in MAO between SLA group and the presence of statistical significance（P<0.05）.Results:Conclusion:1.After treated with MAO high infiltration of TiO₂ membrane structure at eighth weeks,osseointegration is better than that of SLA group.2.After MAO treatment with high invasive structure TiO₂ film with good bone induction effect,it can be used as a replacement method of SLA.3.Micro-CT can analyze the spatial structure of the trabecular bone quickly and effectively around dental implants.