Preliminary Study On Anti-inflammatory Mechanism Of Huperzine A

Huperzine A(HupA)has a strong inhibitory effect on acetylcholinesterase activity that has been used as the therapeutic drug for treating Alzheimer’s disease in Chinese.Besides,HupA is an antagonist of N-methyl-D-aspartate receptor(NMDAR),has a function in enhancing neurotransmitter GABA transmission,and is able to exert the neuroprotective and antiepileptic functions.Currently,the majority of studies focus on its application in Alzheimer’s disease,its anti-inflammatory effect has not been well studied.In this study,we screened the chemical compounds,their intermediate products and natural chemicals for their anti-inflammatory effects in RAW264.7TM cell line by testing its ability to activate NF-κB.The anti-inflammatory candidate compounds were further verified by real-time fluorescence quantitative RT-PCR,immunofluorescence,Western blotting and other molecular biology techniques.Further work on mouse macrophage line-RAW264.7cells and MH-S cells showed that:(1)RAW264.7TM cells were able to quickly and easily screen out anti-inflammatory drugs or intermediate extracts;(2)As the second class of acetylcholine enzyme inhibitors used clinically,HupA has a good anti-inflammatory effect;(3)The anti-inflammatory effect of HupA is mediated by NF-κB-related signaling pathway.Meanwhile,we tested the effect of HupA on anti-inflammatory effects in lipopolysaccharide(LPS)-induced inflammatory mouse model.We found that HupA significantly reduced the expression of inflammatory factors in the inflammatory mice and significantly reversed the LPS-induced histomorphological abnormalities.Many diseases are found to be associated with inflammation,as indicated by the increase in the production of proinflammatory cytokines,such as sepsis that displays typical systemic inflammation symptoms.Our data provide a solid basis and leads to a new pathway for research and development of anti-inflammatory or adjuvant drugs in the future.