Expression And Significance Of STIM1 And TRPC6 In Primary Liver Cancer

ObjectiveTo investigate the expression and significance of STIM1 and TRPC6 in primary liver cancer;The relationship between STIM1 and TRPC6 expression and clinical data and survival prognosis was analyzed;To provide a new entry point for the prevention,diagnosis and targeted therapy for primary liver cancer.Methods1.Flow cytometry: Firstly,40 surgical specimens were collected from primary liver cancer patients diagnosed as hepatobiliary surgery from March 01,2017 to August 31,2017.Flow cytometry was used to detect the expression of STIM1 and TRPC6 in liver cancer patients.2.Immunohistochemical staining and assessment: Through collecting the pathological tissue samples and clinical data of patients diagnosed as primary liver cancer who were admitted to hepatobiliary surgery from July 2011 to July 2012(including the basic physical condition of the patient,the size of the hepatocarcinoma,and the patient’s liver The functional grading,biochemical indicators,and family history,etc.,included 95 cases.The expression of STIM1 and TRPC6 in hepatocellular carcinoma tissues and adjacent tissues was examined by immunohistochemistry and their correlations were analyzed.The relevance of the collected clinical data,and the final statistics of the relationship between STIM1 and TRPC6 and the prognosis of patients;3.Statistical Analysis: Statistical Analysis SPSS 23.0 software was used to compare the categorical variables and the qualitative data was analyzed by Chi-square test or Fisher’s exact test.The Kaplan-Meier method was used for single-factor survival analysis and the survival rate was compared using Log-rank test,multivariate survival analysis using Cox proportional hazards regression model,P<0.05 was considered statistically significant.Results1.Flow cytometry results showed that STIM1 and TRPC6 were expressed in 40 cases of primary hepatocellular carcinoma.The positive rates of the two in primary hepatocellular carcinoma were respectively 62.1% and 51.7%;2.Immunohistochemical analysis showed that the positive rate of STIM1 and TRPC6 in hepatocellular carcinoma was 87.7% and 67.7% in 95 cases of primary hepatocellular carcinoma,and 63.8% and 37.9% in paracancerous tissue(n STIM1=95,P<0.05;n TRPC6=95,P<0.05),suggesting that STIM1 and TRPC6 are differentially expressed in hepatocellular carcinoma and paracancerous tissues,and the expression level in hepatocellular carcinoma is much higher than that in adjacent tissues.High,the difference was statistically significant;3.After analysis,the expression of STIM1 and TRPC6 in hepatocellular carcinoma and the correlation between the two categorical variables were tested by Chi-square test.The results were: 7.501,p=0.006,and the difference was statistically significant(P<0.05).There is a correlation in the expression of HCC;4.Statistical analysis showed that the expression of STIM1 and TRPC6 in primary hepatocellular carcinoma was correlated with tumor size and tumor volume(P<0.05),and the difference was statistically significant.There was a correlation between STIM1 and clinical stage(P < 0.05).The difference was statistically significant,but there was no significant correlation between TRPC6 and clinical stage(P>0.05).The difference was not statistically significant.There was no significant correlation between the expression of STIM1 and TRPC6 and the patient’s gender,age,Child-Pugh classification,differentiation and pathological types(P>0.05).The difference was not statistically significant.5.The univariate analysis of patient survival outcomes showed that high expression of STIM1 and TRPC6(P<0.05),tumor size(P<0.05),clinical stage(P<0.05),and tumor volume(P<0.05)were affecting overall patient outcomes.The prognostic factors of survival time;COX multivariate regression analysis showed that the high expression of STIM1 and TRPC6 was related to the survival and prognosis of primary liver cancer,and the difference was statistically significant(P<0.05).Conclusions1.The high expression of STIM1 and TRPC6 plays an important role in the occurrence and development of primary liver cancer,and there may be a synergistic or synergistic relationship between them.2.The high expression of STIM1 and TRPC6 is an indicator that affects the poor prognosis of primary liver cancer.