ITRAQ Quantitative Proteomics Application In Identifying Serum Biomarkers Of Uyghur And Han T1DM Patients

Objective: To explore the presence of informative serum protein biomarkers of T1 DM patients in Uyghur and Han.Methods: Totally 80 cases of Uyghur and Han T1 DM patients and normal people were selected for this study.This serum proteomic study include 5 groups data comparing.1)the Han T1 DM patients(10 cases)and Han normal people(10 cases);2)Uyghur T1 DM patients(10 cases)and Uyghur normal people(10 cases);3)Han T1 DM patients(10 cases)and Uyghur T1 DM patients(10 cases);4)Han normal people(10 cases)and Uyghur normal people(10 cases);5)Han,Uyghur T1 DM patients and Han,Uyghur normal people;Serum protein of all research objects were extracted.Then 8 iTRAQ markers protein was used,with LC-20 AB liquid phase separation,the peptides were analyzed by LC-ESI-MSMS,the differentially expressed proteins were screened by using IQuant software,at the end GO and pathway enrichment analysis were used to obtain the difference of proteins.Results: 1.Comparing the Han T1 DM patients and Han normal people,509 differentially expressed proteins were screened,including 319 up-regulated and 190 down-regulated proteins;GO enrichment analysis showed that the function of differentially expressed proteins included GTP binding,guanine nucleotide binding,pyrophosphatase activity,hydrolysis enzyme activity;pathway enrichment analysis showed that significant proteins are involved in the pathways of biological adhesion,platelet activation and pyruvate metabolism;2.comparing the Uyghur T1 DM patients and Uyghur normal people,469 differentially expressed proteins were screened,including 287 up-regulated and 182 down-regulated proteins;GO enrichment analysis showed that the function of differentially expressed proteins are included in serine endopeptidase activity,activity of serine endopeptidase inhibitors,regulation of endopeptidase activity,activity of endopeptidase inhibitors;pathway enrichment analysis showed that significant proteins are involved in the pathways of complement and coagulation cascade,ECM receptor interaction,cell adhesion molecule;3.comparing Han T1 DM patients and Uygur T1 DM patients,354 differentially expressed proteins were screened,including 180 up-regulated and 174 down-regulated proteins;GO enrichment analysis showed that the function of differentially expressed proteins are involved in metal ion binding,cation binding,and endonuclease activity;pathway enrichment analysis showed that significant proteins are involved in the pathways of the complement and coagulation cascades,oxidative phosphorylation;4.comparing Han normal people and Uyghur normal people,425 differentially expressed proteins were screened,including 223 up-regulated and 202 down-regulated proteins;GO enrichment analysis showed that the function of differentially expressed proteins are involved in iron binding,heme binding,activity of serine endopeptidase inhibitors;pathway enrichment analysis showed that significant proteins are involved in the pathways of complement and coagulation cascade,tyrosine metabolism;5.comparing Han,Uyghur T1 DM patients and Han,Uyghur normal people,612 differentially expressed proteins were screened,including 461 up-regulated and 151 down-regulated proteins;GO enrichment analysis showed that the function of differentially expressed proteins are involved in ion binding,guanine nucleotide binding,GTP binding;pathway enrichment analysis showed that significant proteins are involved in the pathways of biological adhesion,regulation of actin cytoskeleton,leukocyte migration,platelet activation,ECM receptor interaction.Conclusions 1.A total of 1174 proteins were identified,the repetition rate is 65.5%;2.The main function of differentially expressed proteins are involved in the binding and catalytic activity,and a few of them are involved in the structural molecular activity,transport activity,receptor activity.Metabolic pathway including complement and coagulation cascade,PI3K-Akt signaling pathway,phagocytosis,biological adhesion,may be closely related to T1DM;3.In order to further obtain the specific protein biomarkers that are closely related to T1 DM,the next step is to use MRM technology to further validate the target proteins.