The Expressions And Clinical Significance Of Cathepsin B In Neuroendocrine Tumors In The Digestive Tract

Goals: To detect the expression of Catepsin B protein in the neuroendocrine neoplasm(NEN)in the digestive tract(including esophagus,esophagogastric,stomach,small intestine,colon,rectum,appendix),and to analyze the correlation between the expression of Catepsin B protein and the Clinicopathological parameters such as degree of differentiation,pathological grade,clinical stage,lymph node metastasis and prognosis.In oder to provide the theoretical basis for the clinical diagnosis and prognosis for the NENs in the digestive tract.Materials and methods: We Collected 144 cases from January 1,2010 to December 31,2016,which had been received operation and diagnosed as NENs in the digestive tract by North Sichuan Medical College as an experimental group.And we collected 144 cases normal tissues(with the distance over 5cm from the tumor)as contrast.We detected the Cathepsin B、CgA、Syn、CD56 protein expression in normal tissues and the NENs in the digestive tract by immunohistochemistry.The relationship between the clinicopathological parameters and immunohistochemical staining was analyzed by chi-square test.The Kaplan-Meier survival analysis of the Cathepsin B and the patient’s prognosis was analyzed by the log-rank test.p<0.05 has statistical significance,and p<0.01 has significant statistical significance.Results: The positive expression of Cathepsin B protein is expressed in different degrees in the NENs in the various parts in the digestive tract.The difference in expression of Cathepsin B protein in normal and NENs has significant statistical significance(p<0.01).The protein expression was associated with tumor location,histological differentiation,grade G,clinical stage,survival rate and five year survival rate(p<0.05),but not associated with age group,gender and lymph node metastasis(p>0.05).Conclusions: Cathepsin B may promote the occurrence and development in digestive tract NENs.It is expected to be an effective predictor for judging the location,histological differentiation,pathological grade G,clinical stage and prognosis in NENs of digestive tract.Combining the traditional neuroendocrine markers with Cathepsin B may be beneficial to enhance the accuracy of diagnosis in NENs in digestive tract.