The Research On The Correlation Between The TCM Syndrome And The Liver Pathology Virological Indicators And T Cell Subgroup With Chronic Hepatitis B

1 ObjectiveThis study is attempt to detect the correlation between TCM syndromes of CHB patients with hepatic inflammatory grading,fibrosis stage and T cell subgroup level,hepatitis b 5,and HBV DNA.Patients with chronic hepatitis b were divided into e antigen positive group and e antigen negative group to compare the liver pathological grading of patients.different pathological grades with T cell subgroup level Shows different relationships.2 Methods Reference to CHB diagnostic criteria,put the 122 admitted patientspatients divided into the following five kinds of TCM syndrome: blood stasis resistance winding type,change the type,hygroscopic resistance,splenic kidney Yang deficiency,liver and kidney Yin deficiency type.All the patients were receive liver biopsy.Hepatitis b virus serum markers detection using enzyme-linked immunosorbent method,using fluorescence quantification to test HBV DNA,analysis of CD4+,CD8+ cell subsets and CD4+/CD8+ ratio of peripheral blood by flow cytometry,detection of liver function index by automatic biochemical analyzer,for analyze the relationship between TCM syndromes and T cell subsets,hepatitis B PENTATHLON,HBV DNA,liver inflammation and fibrosisof CHB patients,according to the hbv e of antigen,yin and Yang positive,put them devied into positive group and negative group.3 Results1.Analysis of CHB patients with TCM syndrome type and the correlation of HBsAg,HBeAg,HBV DNA:HBVDNA MOD value(±),the lowest in the spleen and kidney Yang deficiency type(2.83±0.29),damp a nd hot accumulate knot the highest(7.20±0.95),the difference was statistically significant(P < 0.05),spleen and kidney Yangdeficiency type in five card type show the amount of virus in the least,the lowest level of viral replication,HBeAg MOD values change the type(5.364 ± 11.46)has a minimum level of HBsAg drop degree,statistically significant difference(P < 0.05),CHB TCM syndrome type and virology markers have certain contact.2.The detection rate of HBV markers in liver tissues is related to TCM syndrome type,and HBsAg detection rate(96.6%)is the highest in liver tissue.Liver tissue of HBcAg detection rate(72.4%)was lower than those of HBsAg,damp and hot accumulate knot syndrome(69.3%),the highest detection rate,on the statistical analysis,the hepatitis b surface antigen and hepatitis b core antigen detection rate comparison between different TCM syndrome types of TCM had no statistical significance(P > 0.05).This data shows that the detection rate of hepatitis b surface antigen and the detection rate of the core antigen can not be determined by the results of TCM dialectics in patients with chronic hepatitis b.3.TCM syndrome type and the relationship between the liver inflammatory activity and fibrosis,liver and kidney Yin deficiency syndrome in patients with liver inflammation degree is the lightest,but severe fibrosis degree,compared with other type certificate between groups was statistically significant(P < 0.05),change the type of the degree of inflammation and fibrosis in patients with symptoms than the lightest degree than other kind of this syndrome,but no statistical significance compared with damp and hot accumulate knot card,compared with the other three type it statistically,spleen and kidney Yang deficiency patients had serious liver damage,with severe inflammation and fibrosis significantly,and comparison between groups was statistically significant(P < 0.05).4.According to the type of deficiency of traditional Chinese medicine syndrome empirical analysis,the damp and hot accumulate knot and blood stasis resistance collaterals as positive group,the liver and kidney Yin deficiency syndrome,spleen and kidney Yang deficiency syndrome,liver spleen deficiency as deficiency syndrome group,HBeAg positive group MOD values(x±s)to: blood stasis resistance winding(1107.22 ± 579.28),damp and hot accumulate knot(389.37 ± 543.92);Deficiency syndrome group HBeAg MOD values(x ± s): liver and kidney Yin deficiency type(495.02±633.91),spleen and kidney Yang deficiency(9.93±39.79),and to change(210.19±3.61),empirical e antigen level higher than the deficiency syndrome group e antigen level,comparison between groups was statistically significant(P < 0.05),patients with blood stasis resistance winding e antigen,the highest level compared with other syndromes have statistical difference(P < 0.05),spleen and kidney Yang deficiency type e antigen in patients with minimum drop degree,compared with blood stasis type resistance network was statistically difference(P < 0.05);CHB TCM syndrome is related to HBeAg level.5.HBeAg and hepatic pathological grading relationship: the levels of inflammatory activity were highest in patients with G3 level,and the HBeAg level in G4 group was the lowest,but there was no statistically significant difference between the groups(P > 0.05).The degree of fibrosis was the highest in S3 stage HBeAg,and the HBeAg level was the lowest in S4 patients,but there was no statistical difference between the groups(P>0.05).The MOD value of HBeAg and the degree of liver inflammation(G),the correlation coefficient r was 0.11(P>0.05),indicating that the correlation between the two was not strong.The MOD value of HBeAg and the fibrosis stage(G),the correlation coefficient was r 0.14(P > 0.05),indicating that the correlation between the two was not strong.6.HBeAg and hepatic pathological grading relationship: the levels of inflammatory activity were highest in patients with G3 level,and the HBeAg level in G4 group was the lowest,but there was no statistically significant difference between the groups(P >0.05).The degree of fibrosis was the highest in S3 stage HBeAg,and the HBeAg level was the lowest in S4 patients,but there was no statistical difference between the groups(P > 0.05).The MOD value of HBeAg and the degree of liver inflammation(G),the correlation coefficient r was 0.11(P > 0.05),indicating that the correlation between the two was not strong.The MOD value of HBeAg and the fibrosis stage(G),thecorrelation coefficient was 0.14(P >0.05),indicating that the correlation between the two was not strong.7.Inflammatory activity(G)and T cell subgroup relationship: G0-1(17 cases),G2(88 cases)and G3(14 cases),G4(3 cases),patients with inflammatory activity in G2 level,the more the G4 level at least,the degree of G3,CD3+ MOD values(x±s)(65.92±7.71),CD4+ MOD values(x±s)(34.03±10.76),and CD8+ MOD values(x±s)(24.77±8.27),CD4+/CD8+ MOD values(x±s)(1.52±0.66).The values of CD3+,CD4+/CD8+ MOD(x ± s)were the highest in G3 level patients,and the lowest CD8+MOD(x±s)was the lowest,but there was no statistically significant difference in T cell subgroup in G3 patients(P > 0.05).4 ConclusionCHB TCM syndromes and chronic hepatitis B patients with T cell subsets,the index of toxicology,immunohistochemistry,inflammation level,the degree of fibrosis,E antigen positive group and E antigen negative group of patients with TCM syndromes and T cell subsets and virological relationship between differences,different liver pathological grades and T cell subsets have some relationship,in TCM diagnosis and treatment combined with the relevant Western medical indicators.And the patient’s immune function can improve the dialectical accuracy and provide reference for clinical treatment.