| Objective To study the central analgesic mechanism in the treatment of primary trigeminal neuralgia(PTN)in rats,and to provide new thinking and theoretical basis for TCM treatment of primary trigeminal neuralgia.Method 1.90 male SD rats were selected,which were divided into normal group,model group,sham operation group,sunflower receptacle decoction group and camequine group.Among them,the normal group and the sham operation group were excluded,while the remaining rats had the right orbital suborbital neurochronic constriction(ION-CCI).The normal group was given aseptic saline irrigation.At the beginning of the experiment,the model group only cut the skin,but not the inferior orbital nerve.The sunflower receptacle decoction group was treated with low,medium and high dose of sunflower receptacle decoction after the right ION-CCI operation.Carbamazepine(CBZ)group was treated with CBZ after the right ION-CCI operation.2.The pain threshold in the ion-cci side 2 ~ 14 d of rats in the model group was measured by using the von Frey brush in the normal group and the sham operation group.3.Using the method of enzyme-linked immunosorbent(ELISA)determination of rat tissue type induced nitric oxide synthase(iNOS)and substance P(SP)and prostaglandin(PG)and serotonin(5-HT),beta-endorphin(beta EP)and so on the change of neurotransmitter content;4.Immunohistochemistry was used to detect the expression levels of neurotransmitters such as GABA B and calcitonin gene-related peptide(CGRP)in rat brain.Result 1.Changes of neurotransmitters in the model group: the contents of SP,5-HT,beta-EP,CGRP,and GABA B in the model rats were significantly higher in the 5d and 14 d brain tissues than in the normal group and the sham operation group(P < 0.05).In the5 th day,the iNOS content was significantly lower than the normal group and the sham operation group(P < 0.05).In the 14 th day,the iNOS content in the brain tissue was significantly higher than that in the normal group and the sham operation group(P <0.05).There was no statistically significant difference between PG content and normal group and sham operation group in the 14 th day(P >0.05).There was no statistically significant difference between the normal group and the sham operation group in the levels of iNOS,SP,PG,5-HT,and beta-EP in the 5d and 14 d brain tissues(P>0.05);2.Expression of CGRP in rats: there were no statistically significant difference in CGRP protein expression level between the model group and the high,medium and low dose group in the 10 d,20d and 30d(P>0.05).There were no significant difference in CGRP protein expression level between the model group and carbamazepine group in each stage(P>0.05).The expression level of CGRP in the model group was higher than that in the normal group(P < 0.05).The expression level of CGRP protein in the high,medium and low dose group was lower than that in the model group(P < 0.05).3.The expression of GABA B: rats model and sunflower receptacle decoction of 5d low dose group,low dose group,and in 20 d,10 d,30 d of sunflower receptacle decoction of high,medium and low dose group of GABA B protein expression level of comparative differences had no statistical significance(P > 0.05);The expression level of GABA B protein in the model group was higher than that in the normal group(P < 0.05).The expression level of GABA B protein in the high and medium dose group was lower than that in the model group(P < 0.05).The expression level of GABA B protein in the high and medium dose group was lower than that in the model group(P < 0.05).4.Changes in 5-ht content in rats: there were no statistically significant difference in5-HT content between the model group and the 5d high,medium and low-dose group of the model group(P>0.05).There was no statistically significant difference in 5-HT content between the model group and the 20 d high,medium and low dose group(P>0.05).There were no statistically significant difference in 5-HT content between the model group and the 30 d high,medium and low dose group(P>0.05).The content of 5-HT in the high,middle and low-dose group was lower than that in the model group(P < 0.05).The content of 5-HT in the 10 d of carbamazepine group was lower than that in the model group(P < 0.05).5.The changes in the contents of beta-EP in rats were not statistically significant(P>0.05)in the model group and in the 10 d high,middle and low dose group of the model group and the low-dose group.There was no statistically significant difference between the model group and the high,medium and low-dose group in the 20 d high,middle and low dose group(P>0.05).There was no statistically significant difference between the model group and the high,medium and low-dose group(P>0.05).There was no statistically significant difference between the model group and the carbazepine group(5d,10 d,20d,and 30d)(P>0.05).In the 5d high,medium and low dose group,the contents of the high,medium and low dose group were higher than that in the model group(P < 0.05).6.There was no statistically significant difference in the content of iNOS,PG and SP in the brain tissues of the rats in the group of rats after treatment of the model group and different doses in different time periods(P>0.05).There was no significant difference in the contents of iNOS,PG and SP in rats in different time periods in the model group and carbamazepine group(P>0.05).Conclusion 1.By using ION-CCI,it can successfully establish the primary trigeminal neuralgia model of rats,which can lead to changes in the neurotransmitter content of iNOS,SP and PG of the central nervous system.2.Sunflower receptacle decoction to treat rats PTN central analgesia mechanism and regulation of the brain 5-HT,beta EP,GABA B,CGRP and other neurotransmitters on(among them,the neurotransmitters 5-HT lower after treatment,speculated that this mechanism may be related to sunflower receptacle decoction on damage morereceptor subtype).3.One of the effective mechanisms for the treatment of PTN in rats was related to the regulation of neurotransmitters such as 5-HT,beta-EP and other neurotransmitters in the brain. |
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