The Comparative Study Of Biological Behaviors Of Osteoblasts On SLA And Double Acid Etched Titanium Surfaces

Objective: To comparatively evaluate the influences of sandblasting acid etched(SLA)and double acid etched titanium surfaces on biological behaviors of osteoblasts.Methods: Sandblasting acid-etching and double acid-etching were performed respectively on pure titanium surfaces.The pure titanium surface(Ti)was used as the control group;sandblasting acid etched surface(Ti-SLA)and double acid etched surface(Ti-DA)were used as two experiment groups.The micro-topography,hydrophilicity and elemental compositions of different titanium surfaces were observed and analyzed via scanning electron microscopy(SEM),contact angel test and X-ray photoelectron spectroscopy(XPS).MC3T3-E1 cells were seeded on samples of three groups to investigate the effects of different titanium surfaces on biological behaviors of osteoblasts.Results: SEM observation showed that more uniform dense micro-porous structure was formed on the Ti-DA surface can form compared with the Ti-SLA surface.There were no evident differences of surface contact angles among three different groups.XPS analysis revealed that trace aluminum element had been remained on the Ti-SLA surface.Adhesion and proliferation abilities of osteoblasts on samples of the Ti-SLA and the Ti-DA surfaces showed no obvious differences,while Ti-DA group significantly promoted osteoblast differentiation.Conclusion: Compared with the SLA titanium surface,the double acid etched titanium surface exhibited more uniform dense micro-topography without aluminum residue,and effectively promoted osteoblast differentiation.