| Fatigue is a common physiological phenomenon in modern society.Fatigue can be caused by excessive fatigue and no obvious cause.Fatigue can not be recovered in time,which can lead to a variety of chronic diseases and harm the health of the human body.The researchers are aware of the causes of fatigue from different aspects and form several different theories.This study is related to the theory of accumulation and the theory of free radicals.At this stage,the treatment of fatigue in traditional Chinese medicine begins to attach importance to harmonization,and no longer emphasizes the formation of fatigue for pure deficiency,and gradually comes out of the misunderstanding of supplement.In this experiment,the theoretical foundation of"jinguiyaolue.Blood arthralgia consumptive disease"on the understanding and treatment of consumptive disease on the selection of treatment body fatigue Xiaojianzhong decoction,through the experimental study on the anti fatigue mechanism.Purpose:1.through literature review,understanding and understanding of Chinese medicine treatment of fatigue,analysis of legislative principle of Xiaojianzhong Decoction of yin and Yang,to explore the essence of anti fatigue Xiaojianzhong decoction.2.through animal experiment,establish exhaustive model,clear effect of Xiaojianzhong Decoction on lipid metabolism,explore the peroxidation in energy metabolism and lipid’s promoting role.3.by Western blot test,observation of Xiaojianzhong Decoction on oxidative stress reaction in mice model of fatigue on the Keap1-Nrf2-ARE signal transduction pathway in the role of.Method:60 mice were randomly divided into control group(N group),model group(E group)and Xiaojianzhong Decoction low dose group(SL group),Xiaojianzhong Decoction dose group(SM group),Xiaojianzhong Decoction high dose group(SH group),8 rats in each group.E,SM,SH group and SL group respectively for 10 days of exhaustive loaded swimming experiment(exhaustive standard:the mice in the water rotation,motor coordination decreased obviously,flooded the nose,body sink,and again surfaced time over 10s,is brought back to be raised up),and according to the 200mg.Kg~-11 D~-11 dose groups were gavaged with normal saline and the corresponding dose of Xiaojianzhong decoction.After tenth days,each group was loaded with weight swimming.After the exhaustion was exhausted,the mice were killed,and the materials were dissected and measured.The mice swimming time,the ratio of serum urea assay(UREA),creatinine(CREA),lactate dehydrogenase(LDH)content,and muscle glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),malondialdehyde(MDA),muscle glycogen(GLY)expression in each group of mice shares;four head muscle Keap1and Nrf2 protein were determined by Western-blot method.Result:1.Xiaojianzhong decoction can significantly improve the swimming time of mice(p<0.05),improve the exercise tolerance of mice;2.after exercise,the contents of UREA,CREA and LDH in the E group were significantly higher than those in the N group(p<0.05),indicating that the metabolites of protein metabolism in the body after exercise increased and the metabolites accumulated,and that the fatigue state had been caused.Compared with the E group,the content of UREA in the SL group decreased significantly(p<0.01),and the UREA content in the SM group had a statistically significant difference(p<0.05),and the CREA content in SL group was significantly lower than that in the E group(p<0.01)and the SM group content decreased significantly.The content of CREA in group SH and the content of LDH in group SM had no significant difference(p>0.05)in E group(p>0.05),indicating that middle and low middle dosage of small construction could better remove the metabolites of the body and improve the energy metabolism of the body.3.After exercise,the content of GSH-Px and GLY in the E group was significantly lower than that in the N group(p<0.01).The content of SOD in the E group decreased and the MDA content increased(p<0.05),indicating that the free radicals and lipid peroxidation products in the body increased and the energy substances in the body decreased.Compared with the E group,the content of GSH-Px in group SL and SH increased(p<0.05),and the content of SOD in group SH increased significantly(p<0.05),and MDA content in SL and SM groups decreased significantly(p<0.01).Meaning(p>0.05).It shows that low and high doses of Xiao Jianzhong Decoction can promote the production of antioxidant enzymes and reduce the accumulation of peroxide products,but can not provide energy substances.4.After exercise,the expression of Keap1 protein in the E group was higher than that in the N group(p<0.05).Compared with the E group,the Keap1 protein expression in the SL and SH groups decreased significantly(p<0.05),and the SL group was significantly different(p<0.01).The expression level of Nrf2 protein in M and SH groups was increased(p<0.05).It shows that Xiao Jianzhong Decoction can be more able to dissociate Keap1 and downstream protein at low and high doses,activate Nrf2 protein into nucleus and ARE to activate Keap1-Nrf2-ARE signal pathway,combining with the result of 3,it shows that Xiao Jian Zhong decoction can effectively combat the oxidative stress reaction of the body.Conclusion:The results confirm that Xiao Jianzhong Decoction can significantly improve the exercise endurance of mice;it can improve the body’s energy metabolism and accelerate the clearance of serum metabolites to promote glycolysis;it can reduce the oxidation products resulting from fatigue and participate in the Keap1-Nrf2-ARE signaling pathway,activate the antioxidant protease and remove the excess from the body.It can prevent or slow down the lipid peroxidation,thereby alleviating the adverse effects of fatigue,and has the function of anti fatigue. |
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