| Small ubiquitin-related modifier protein(SUMO)modification is a newly discovered post-translational modification of proteins,and its modification process is very similar to ubiquitin modification.Like the deoxyuridine modification,the SUMO modification is mainly performed by a family of SUMO specific protease SUMO-special proteaseses(SENPs).A large number of studies have shown that the abnormal expression of SENPs family proteins is closely related to the occurrence and development of various human malignant tumors such as liver cancer,bladder cancer,breast cancer and so on.However,the mechanism of action of SENPs on malignant glioma is rarely reported,and the specific mechanism is still unclear.Therefore,the effect of SENPs on glioma and its related mechanism were investigated.In this experiment,we first investigated the expression of SENPs family members in normal human brain tissues.The results of Western blot showed that only a small amount of SENP1 was expressed in human normal brain tissues.Then,the Western blot analysis of glioma tissues was carried out.The results showed that the expression of SENP1,SENP2 and SENP6 in malignant gliomas was significantly higher than that in normal brain tissues(P < 0.05).In order to confirm the above results,we selected Cos7 cells as control group and glioma LN443 and U343 cells as experimental groups.The above-mentioned cells were cultured and the samples were obtained by subculture.Results the expression of SENP2 and SENP6 in Cos7 cells was detected by Western blot,but the expression of SENP1 was not obvious.The expression of SENP1,SENP2 and SENP6 in glioma LN443 and U343 cells was significantly higher than that in the control group(P < 0.05).In conclusion,compared with normal brain tissues,SENP1,SENP2 and SENP6 in glioma tissues and cells were significantly higher than those in normal brain tissues(P <0.05).Therefore,we speculate that the overexpression of SENPs in gliomas may be involved in the genesis and development of gliomas. |
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