| BACKGROUND:Colorectal cancer is a malignant gastrointestinal cancer that is harmful to human,s health.In 2012,about 1.36 million new cases of colorectal cancer were diagnosed in the worldwide,and the number of deaths was as high as 690,000.In2010,there were more than 270,000 new cases of colorectal cancer in China and the number of deaths is 130,000.In clinical practice,the treatment failure of colorectal cancer is often attributed to lymph node metastasis of tumor cells,distant metastasis of blood and recurrence of tumor.With the rapid development of molecular biology technology,the pathogenesis of colorectal cancer is gradually being recognized,and new oncogene and tumor suppressor genes are involved in the occurrence,development and evolution of colorectal cancer.Human genome research shows that protein-coding genes account for less than 2 percent of the genome,and the number of encoded genes in the genome exceeds our expected non-coding RNAs(Lnc RNA).Lnc NRA is a class of transcriptional copies of more than 200 nucleic acid molecules,which are not only involved in the coding of proteins,but also participated in many biological processes in the form of RNA.In recent years,a large number of studies have shown that the expression abnormality of Lnc RNA is closely related to the occurrence and development of various human tumors.In recent years,a large number of studies have shown that the expression abnormality of Lnc RNA is closely related to the occurrence and development of various human tumors..In the present project,we first analyzed the expression of Lnc RNA MIR31 HG in CRC,and and further determined the clinical significance,and the function role of MIR31 HG on CRC cells,which may help us to better understand the pathogenesis of CRC,and further provide new therapeutic target for the treatment of CRC.OBJECTIVE: The aim of this study is to explore the expression of Lnc RNA MIR31 HG in colorectal cancer,and its clinical function and the role of MIR31 HG knockdown on cell migration and invasion in colorectal cancer cells.METHODS: The expression of Lnc RNA MIR31 HG was evaluated by q RT-PCR assy in colorectal cancer and corresponding non-tumor tissues.Lnc RNA expression profile was downloaded from pubmed GEO and TCGA database.Kaplan-Meier method and log-rank test were used to estimate the survival rates.The effect of MIR31 HG knockdown was confirmed by q RT-PCR assay.The functional role of MIR31 HG knockdown on CRC cell proliferation and migration were determined by MTT and transwell assay.RESULTS: The expression of Lnc RNA MIR31 HG in CRC tumor tissues(4.16± 0.3)was high than it in corresponding non-tumor tissues(2.31 ± 0.22),n = 20,P <0.001.The expression of Lnc RNA MIR31 HG in CRC tumor tissues(11.23 ± 1.05)was high than it in corresponding non-tumor tissues(7.97 ± 0.78)in GDS2947 profie,n = 32,P value < 0.001.MIR31 HG expression of CRC tumor tissues in the profile of GDS4379(n = 62)and GDS4516(n = 104)are 4.3 ± 0.12 and 4.45 ± 0.04,respectively.Patients with high MIR31 HG expression had poorer 5 year overall survival than those with low MIR31 HG expression,HR = 1.52;95% CI: 1.02-2.25;P = 0.0387.MTT assay shown that,in SW480 cells the A Values of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group in 48 h were 0.43 ± 0.03,0.44 ± 0.04 and 0.68 ±0.04,respectively(P = 0.0073,P = 0.0125).The A Values of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group in 72 h were 0.733 ± 0.03,0.83 ± 0.05 and 1.19 ±0.04,respectively(P = 0.0008,P = 0.006).The A Values of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group in 72 h were 1.00 ± 0.07,1.06 ± 0.09 and 1.45 ±0.06,respectively(P = 0.0073,P = 0.0226).In Lovo cells,the A Values of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group in 48 h were 0.36 ± 0.02,0.38 ±0.04 and 0.57 ± 0.06,respectively(P = 0.0318,P = 0.0591).The A Values of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group in 72 h were 0.60 ± 0.01,0.68 ±0.05 and 0.88 ± 0.06,respectively(P = 0.0124,P = 0.068).The A Values of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group in 96 h were 0.95 ± 0.05,1.04 ±0.08 and 1.42 ± 0.09,respectively(P = 0.0107,P = 0.0377).Furthermore,transwell assay shown that,in SW480 cell the membrane cell numbers of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group were 32 ± 2,39 ± 3and 102.3 ± 7,respectively(P = 0.0007,P = 0.0013).In Lovo cells,the membrane cell numbers of si-MIR31 HG #1,si-MIR31 HG #2 and si-NC group were 29 ± 2,39± 4 and 95 ± 5,respectively(P = 0.0003,P = 0.0008).CONCLUSION: Lnc RNA MIR31 HG is highly expressed in colorectal cancer,and correlates with poor prognosis and adverse biological function.These results indicate that MIR31 HG plays a critical role in CRC progression,and may provide a new therapeutic target for CRC. |
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