The Application Of I-PRF Compound Bio-Oss Bone Powder In Tooth Extraction Site Preservation

Objectives This study aims to study the effect and the mechanism of injectable rich platelet fibrin(injectable platelets rich-fibrin,i-PRF)compound Bio-Oss on alveolar bone remodeling in animals after tooth extraction.Methods The 36 New Zealand white rabbit(3-months-old,male and female)wereselected.The chin left incised tooth of the animals was extracted under general anesthesia.The animals were divided into four groups according to the different fillers in the tooth extraction socket: i-PRF+Bio-Oss group,PRF+Bio-Oss group,Bio-Oss group and the blank control group.After 1 week,4 weeks,12 weeks,the animals were executed and the slice of alveolar bone prepared.The HE staining was used to analyze the new bone formation in the extraction wound.The Immunohistochemical method was adopted to detected the protein levels of osteoprotegerin(OPG)and osteoclast differentiation factor RNAKL.The real time quantity PCR(RT-q PCR)was used to detected the m RNA levels of OPG,RNAKL and osteogenic related gene Runx2.Results 1 HE staining showed that the quality and quantity of osteogenesis of iPRF+Bio-Oss group were superior to those of other groups both at the 4 weeks and 12 weeks.The quality and quantity of osteogenesis ofeach group at the 4 weeks were superior to those at the 12 weeks.2 The immunohistochemical results indicated that the protein level of OPG of i-PRF+Bio-Oss group was significantly higher than that of other groups(P<0.05),the OPG protein level of each group at the 4 weeks was markedly higher than that at the 12 weeks(P<0.05).On the other hand,the protein level of RANKL of iPRF+Bio-Oss group was significantly lower than that of other groups(P<0.05),and the RANKL protein level of each group at the 4 weeks was markedly lower than that at the 12 weeks(P<0.05).3 The RT-q PCR results confirmed that the OPG m RNA level of iPRF+Bio-Oss group was markedly upregulated compared with that of other groups at 1 week,4 weeks and 12 weeks,respectively(P<0.05).The upregulation effect of each group at the 4 weeks was most obvious(P<0.05).On the other hand,the RANKL m RNA level of i-PRF+Bio-Oss group was significantly decreased compared with that of other groups at 4 weeks and 12 weeks,respectively(P<0.05),whereas there was no difference among the four groups at 1 week(P>0.05).Meanwhile,the Runx2 m RNA level of i-PRF+Bio-Oss group was significantly higher than that of other groups at 1 week and 4 weeks,respectively(P<0.05),whereas there was no difference among the four groups at 12 week(P>0.05).The Runx2 m RNA level of each group was the highest at the 4 weeks,but decreased at the 12 weeks(P<0.05).Conclusions 1The function of i-PRF compound Bio-Oss bone powder of promoting the regeneration of bone tissue is superior to PRF compound Bio-Oss,and could be considered as a better material for the preservation of tooth extraction site.2 The i-PRF compound Bio-Oss bone powder has the better effects on upregulation of OPG and Runx2,as well as downregulation of RANKL,than PRF compound Bio-Oss.