The Relationship Between ZEB1 And Its Related Regulatory Factors NF-kb, E-Cadherin And The Pathogenesis Of Colorectal Cancer

Objectives Immunohistochemical SP method was used to detect ZEB1,NF-kb,and ECadherin in colorectal cancer tissues,colorectal benign lesions(atypical hyperplasia or polyps detected by colonoscopy),and normal mucosa adjacent to colorectal cancer.The expression level of Cadherin and the relationship between ZEB1,NF-kb,E-Cadherin and various clinicopathological parameters of colorectal cancer were analyzed,and the relationship among ZEB1,NF-kb and E-Cadherin were analyzed.To investigate the expression of ZEB1,NF-kb and E-Cadherin in colorectal cancer tissues and its relationship with the progression of advanced colorectal cancer,and further analyze its mechanism of action in tumor proliferation and metastasis for patients with colorectal cancer.Provide helpful help in diagnosis,treatment,and prognosis.Methods Immunohistochemical SP technique was used to detect 72 sporadic human colorectal cancer tissues,45 benign lesions(indicated by colonoscopy for atypical hyperplasia or polyps),and 45 adjacent normal tissues(from colorectal cancer surgical specimens).The expression of ZEB1,NF-kb,And E-Cadherin in the normal mucosa of the stump,and the relationship between the three clinicopathological parameters of colorectal cancer were analyzed,and ZEB1,NF-kb,and E-Cadherin were other performed.Correlation analysis.Results The positive rates of 1ZEB1 and NF-kb were significantly higher in the cancerous tissue group(73.63%,76.4%)than in the benign colorectal tissue group(17.8%,33.3%)and normal tissue adjacent to the cancer(13.3%,26.7%).All were statistically significant(P<0.05);the positive expression rate of 2E-Cadherin was significantly higher in benign colorectal tissue and adjacent normal tissue(93.3%,95.6%)than in colorectal tissue(11.11%).Statistical significance(P<0.05).The positive rates of 3ZEB1 and NF-kb were significantly higher in colorectal cancer III+IV(88.9%,91.7%)than in colorectal I+II(58.3%,61.6%);ZEB1,NF-kb The positive expression rate in the poorly differentiated group(91.3%,91.3%)was significantly higher than in the high school differentiation group(65.3%,69.4%);the positive expression rate of ZEB1,NF-Kb in the lymph node group(91.7%,91.7%)The positive expression rates of ZEB1 and NF-Kb were significantly higher in the invaded serosal group(91.4%,88.6%)than in the non-invasive serosa group(56.8%,significantly higher than those in the no lymph node group(55.6%,61.1%).64.9%);The above differences are statistically significant(P<0.05).4 The positive expression rates of ZEB1,NF-kb and E-Cadherin in colorectal cancer tissues were not significantly different among different ages and genders(P>0.05).5 ZEB1 and NF-kb were positively correlated in colorectal cancer tissues(r=0.992,P=0.00).ZEB1 and E-cadherin,NF-Kb and E-cadherin expression were negatively correlated in colorectal cancer tissues.(r=-0.625,P=0.00;r=-0.529,P=0.00).Conclusions 1 ZEB1 and its related regulatory factors NF-kb,E-Cadherin are closely related to the invasion and invasion ability of colorectal cancer.2 The high expression of ZEB1 and NF-kb in human colorectal cancer tissues,and the low expression of ECadherin in human colorectal cancer tissues are closely related to the progression of advanced colorectal cancer tissues,especially those with advanced colorectal cancer.The proliferation and transfer are closely related.3 ZEB1,NF-kb,E-Cadherin may play an important role in promoting the pathogenesis of colorectal cancer.4 Regulatory factor NF-kb and the transcriptional repressor gene ZEB1 have a synergistic effect,and both have the effect of down-regulating the expression of E-Cadherin factor,thereby promoting the occurrence,invasion and metastasis of colorectal cancer tissues.