Application Of Mechanical Loading In Rehabilitation Of Hypertensive Rats

Objective: Hypertension refers to the main feature of increasing body-circulation arterial blood pressure,which can be accompanied by the clinical syndromes of heart,brain,kidney and other organ functions or organic damage.It is also the most important risk factor of cardiovascular and cerebrovascular diseases and seriously endangers people’s health.Studies have confirmed that aerobic exercise can lower blood pressure.Our self-developed pulsed mechanical stimuli mechanically stimulates synovial joints and spine in rats at certain loading frequencies to simulate physical aerobic exercise.The purpose of this experiment was to observe mechanical loading has antihypertensive effects on hypertensive rats and it has protective effects on important target organs such as blood vessels,heart,and kidneys.Method:1)In this experiment,50 six-week-old female Sprague-Dawley rats weighing200 g were divided into control group 10,study group 40 by using random number table.The study group was given high-salt and high-fat diet induction for six weeks.The noninvasive tail artery calorimetry was used to monitor the blood pressure(1 /week).Finally there were 22 rats with a sustained systolic blood pressure above158 mm Hg.20 of 22 hypertensive rats were randomly selected and randomly divided into model group and treatment group(n = 10).Rats in the treatment group were subjected to mechanical loading every day for 5 Hz,2N,5 min / day for 6 weeks.The body weight,body fat content,BMI and blood pressure of the rats in each group were recorded.The degree of vascular,heart and kidney injury was observed by HE staining.2)The renovascular hypertensive models were produced by classic two-kidney-one hypertensive renal hypertension rat model.12 rats were randomly divided into model group and treatment group,at the same time a sham operation group was set up.The rats in the treatment group were subjected to mechanical loading every day.After 6 weeks,the blood pressure of each group was recorded to calculate the kidney/body mass index.The renal damage and renal vascular remodeling were observed under light microscope,TNF-α content.Results:The successful rate of hypertension in study group was 56%.The study group had a 56% success rate of hypertension.After 6 weeks of modeling,the body weight, fat mass,BMI value,systolic blood pressure,diastolic blood pressure,and average caudal arterial pressure of the model group were significantly increased compared to the control group,and the values after mechanical loading treatment were significantly reduced.Histological observations showed that significant damage occurred in the blood vessel,heart,and kidney of the model group,which was mainly manifested in the thoracic aortic wall area,blood vessel wall thickness,vascular wall/vessel cavity ratio,ventricular wall thickness,and myocardium.Increased cell area and increase in renal index,renal interstitial inflammatory cell content.After 6weeks of mechanical loading,the degree of damage to blood vessels,heart and kidneys was significantly improved compared to the model group.2)Compared with the control group,the average caudal arterial pressure increased significantly in the model group,and the blood pressure of the rats after mechanical loading was significantly lower than that of the model group;the glomerular injury index and interstitial inflammation of the clamped and unclamped sides of the model group rats The number of cells and the content of IL-6 and TNF-α were significantly higher than those in the control group,and they were significantly decreased after mechanical loading.Conclusion:In this experiment,high salt and high fat diet and two kidneys and one clip were used to induce hypertension model successfully.The results showed that mechanical loading could significantly reduce the blood pressure of two kinds of hypertensive rats.2)Mechanical loading also has a good curative effect on the rehabilitation of obesity with high salt and high blood pressure in rats,at the same time it can improve the blood vessels,heart and kidneys caused by hypertension.3)Mechanical loading could improve glomerular injury induced by renal hypertension and renal interstitial inflammation,reduce the number of interstitial inflammatory cells and the content of IL-6 and TNF-α.4)This topic provides new ideas for the treatment of cardiovascular diseases.