Study On The Interaction Of Pillar[5]arene-based Imdazolium Salt With Quinine Sulfate And BSA And Quantitative Detection Of Ions By Spectroscopic

Objective:Research the interaction mechanism of pillar[5]arene-based imdazolium salt（WP5）and quinine sulfate（QS）.Improve the research method of host-guest interaction by Spectroscopy.Research the interaction mechanism of WP5 and BSA,and provide reference for the application of WP5 as a drug carrier.Perform the quantitative detection of Fe³⁺and F^-by the“OFF-ON”fluorescence spectroscopy of QS.Study the mechanism on the fluorescence“OFF-ON”of QS.Methods:（1）Calculate the quenching constants of WP5 and QS at different temperatures by fluorescence spectroscopy.And researche the UV-vis absorption differential spectroscopy.（2）Research the interaction mechanism of WP5 and BSA by fluorescence spectroscopy and UV-Vis absorption spectroscopy at different temperatures.Calculate the binding force,the number of binding sites and the related thermodynamic parameters.Study the main force.Study the effect of WP5 on the conformation of BSA amino acid residues and by synchronous fluorescence spectroscopy to investigate the possible biological toxicity of WP5.（3）Quantitatively detecte the Fe³⁺by the fluorescence quenching spectroscopy of QS,and quantitatively detecte the F^-by the fluorescence enhancement spectroscopy of QS-Fe³⁺.Results:（1）The fluorescence quenching mechanism of QS was confirmed to be dynamic quenching by fluorescence spectroscopy and UV-Vis absorption spectroscopy.The interaction of WP5 and QS might be due to the increase of molecular diffusion in the excited state.（2）The fluorescence quenching mechanism of BSA was confirmed to be static quenching by fluorescence spectroscopy and UV-Vis absorption spectroscopy.The binding constant at 293K is 2.810×10⁴ L/mol and at 303K is 9.649×10⁴ L/mol.The binding ratio is 1:1.The binding process is spontaneous.The main force is hydrophobic force.By synchronous fluorescence spectroscopy,WP5 was proved to be a certain impact on the conformation of BSA amino acid residues,which might be biological toxicity.（3）The quantitative detection of Fe³⁺by QS was achieved with a detection limit of 1.0×10^-55 mol/L and a linear range of 4×10^-55 mol/L to 8×10^-44 mol/L.The quantitative detection of F^-by QS-Fe³⁺was achieved with a detection limit of 4.4×10^-55 mol/L and a linear range of1.2×10^-44 mol/L to 2.4×10^-33 mol/L.Conclusion:In this thesis,WP5 was synthesized.The interaction of WP5 and QS was studied by spectroscopic methodology,which improved the research methods of spectroscopy on host-guest interaction.The interaction of WP5 and BSA was studied,and its possible biological toxicity was found,which provided reference for the future application of WP5 in drug carrier.The quantitative detection of Fe³⁺and F^-was realized by the“OFF-ON”fluorescence spectroscopy of QS.The mechanism of fluorescence“OFF-ON”was“Electron/Hole Radiation Recombination-Electron/Hole non-Radiation Recombination-Electron/Hole Radiation Recombination”.