| [Objective] 1.Mycoplasma genitalium(Mg)is a kind of anogenital pathogen that found out in recent years which can lead to sexually transmitted diseases.Generally the infection status of Mg among people is still limited researched.The aim of this study is to find out the infection status of STD clinic patients in Tianjin Medical University General Hospital,and also to provide the premise and support for Mg infection control.2.Chlamydia trachomatis(CT)is a usual pathogens of nongonococcal urethritis and also can lead to serious consequences.In the detection of CT,different specimens can be selected according to different detection methods.Polymerase Chain Reaction(PCR)is a common detection method due to high sensitivity,and the common specimens are urethral/endocervical swabs and urine.By detecting CT in different specimens by PCR technology,the results can finally concluded to a new concept of how to detect Mg in a right way.[Method] 1.309 clinic patients’ data from 2017 September to 2018 March were collected.Among of all,180 patients(135 males,45 females)were suspected urogenital tract infected.Taking their genital secretions swab(urethra/ A cervical swab)and use PCR to detect Mycoplasma genitalium Deoxynucleotide(Mg DNA).129(95 males,34 females)cases of ordinary physical examination were set up as healthy group and also carry out PCR detection of Mg.At last,the test results will be counted,compared and analyzed.2.116 patients(72 male,44 female)are collected in Tianjin Medical University General Hospital,and all the clinics are suspected genitourinary tract infection.Male is tested by urethral swab specimens,and female by cervical swab specimens.All their urine are kept afterwards.By using the Roche kit and Roche cobas4800CT/NG detector,the positive rate of CT in different specimens can be detected by PCR.And the final results of different specimens can be compered and analyzed.[Result]1.There were 13(7.22%)cases of Mg positive in 180 patients with suspected urogenital tract infection in STD clinic,including 9(6.67%)males and 4(8.89%)female.The Mg positive rate in different gender had no statistically significant difference(χ2 =0.25,P>0.05).78 patients age from 19~30,9(11.53%)of them were Mg positive.Age from31~40 is 49 patients,2(4.08%)positive cases was found.41~71 years old range is 53 cases,including 2(3.77%)positive cases.The difference of Mg positive rate from each group had no statistically significant(H=3.297,P>0.05).In 129 healthy control group,2(1.55%)cases of Mg positive were compared with 13 of STD patients in clinic,the difference had statistically significant(χ2 =5.234,P<0.05).2.Among the 116 patients who were examined in the STD clinic,the results of CT examination in urethral /cervical swab and urine were: 16(13.79%)、15(12.93%).The positive rates were compared and the difference had no statistically significant(χ2 =0.037,P>0.05).[Conclusion] 1.Infection of Mg in STD patients exists in our hospital,and the positive rate is higher than in normal healthy people.It didn’t show the gender differences.In the age range,the sexually active group is higher potential to be infected.But overall it didn’t show the age difference.Mg also exist in normal healthy people.We should focus on the Mg detection among STD patients and also the healthy group.And doctors should pay attention on the treatment of Mg infection.2.The results of CT detection are almost same by detecting on urine samples and the urethral/cervical specimens.And the detection rates are both high.So for the detection of CT by PCR fluorescence,we can consider using urine specimens instead of traditional urethral/cervical specimens.It is easy and quick to obtain urine specimen and can relieve patient’s pain.It is easy for patients to accept,and also provides new ways for patients with or without symptoms.The higher detection rate of CT also shows that we should pay more attention to the monitoring and giving timely treatment of CT infection. |
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