Effect Of Tea Polyphenols On Intestinal Flora,Intestinal Enzyme Activities And Short Chain Fatty Acids In Mice

Objective: Mice intestinal dysbacteriosis model was established,To study the effects of tea polyphenols on intestinal microflora,intestinal enzyme activity and intestinal short-chain fatty acids in mice,and to explore the mechanism of tea polyphenols in vivo.Method:(1)Infusing mice with Lincomycin hydrochloride injection to establish a mice intestinal flora dysbacteriosis model.(2)Using 16SrDNA technology study the regulation effect of tea polyphenols on intestinal microflora in mice.(3)Using Enzyme-linked Immunosorbent assay technology to study the effect of tea polyphenols on intestinal enzyme activity in mice.(4)Using Gas Chromatography to determined the effect of tea Polyphenols on intestinal short-chain fatty acid content in mice.Results:(1)Compared with the normal control group,the intestinal flora diversity of the model mice decreased,the bacterial structure changed,and the abundance of the main Phylum Bacteroides in the gut was significantly decreased(p<0.05),and the abundance of Escherichia/Shigella was significantly increased(p<0.05).(2)Compared with the model control group,after oral administration of tea Polyphenols,In the small intestine,the diversity and richness of bacterial increased and the relative abundance of Proteobacteria decreased significantly(p<0.05).The relative abundance of Bacteroidetes and Firmicutes were increased,and the relative abundance of Escherichia/Shigella was significantly decreased(p<0.05).In the cecum,the diversity and richness of the bacterial flora were decreased,the relative abundance of Bacteroidetes was significantly increased(p<0.05),and the relative abundance of the Firmicutes was significantly decreased(p<0.05),and the relative abundance of the Proteobacteria was increased slightly(p>0.05).In the colon and large intestine,the diversity and richness of bacterial flora were decreased,the relative abundance of Bacteroidetes was increased significantly(p<0.05),the relative abundance of Firmicutes was decreased significantly(p<0.05),and the change of Proteobacteria was not obvious.(3)Compared with the model control group,changes of endogenous enzymes in the intestine of mice after oral administration of tea Polyphenols was as follows: In the small intestine,Trypsin activity was increased significantly(p<0.05),Lipase activity was increased,and high-dose TP can increase Lipase activity significantly(p<0.05),and Amylase activity was significantly decreased(p<0.05).In the cecum,the activity of Trypsin was increased significantly(p<0.05),Lipase activity was decreased significantly(p<0.05),and Amylase activity was decreased,Amylase activity was decreased significantly in the high and middle dose groups(p<0.05),while in low dose group Amylase activity was decreased but not significantly(p>0.05).In the colon,the activity of Trypsin was increased,and the Trypsin activity in the high-dose and low-dose tea Polyphenols groups was increased significantly(p<0.05),and the activity of Amylase and Lipase decreased significantly(p<0.05).In the large intestine,the activity of Amylase and Lipase was decreased significantly(p<0.05),Trypsin activity was increased,and the Trypsin activity was increased significantly in the high-dose group and the low-dose group(p<0.05).The changes in intestinal exogenous enzymes were as follows: In the small intestine,the activity of Cellulase was decreased significantly(p<0.05),and the dose-effect relationship was observed;the activity of Xylanase was increased significantly(p<0.05),and the effect of medium dose tea Polyphenols was the best.In the cecum,the activity of Cellulase was decreased significantly(p<0.05),and the activity of Xylanase activity was increased significantly(p<0.05).In the colon,the change of Cellulase activity was not obvious,the Xylanase activity was increased,and the effect of low dose tea polyphenols was significantly(p<0.05).In the large intestine,the activity of Cellulase was decreased significantly(p<0.05),and the activity of Xylanase activity was increased significantly(p<0.05).(4)Compared with the model control group,In the small intestine,the content of Acetic acid,Butyric acid and Isovaleric acid increased significantly after intragastric administration of tea Polyphenols(p<0.05).Propionic acid content in the middle and high dose groups was increased significantly(p< 0.05),low-dose tea Polyphenols increased the content of Propionic acid(p> 0.05),Isobutyric acid and Valeric acid content were increased(p> 0.05).In the cecum,the contents of Acetic acid,Isobutyric acid and Isovaleric acid were increased significantly(p<0.05),and the content of Valeric acid was increased.The middle dose of tea Polyphenols increased Valeric acid content significantly(p<0.05),Propionic acid and Butyric acid content were decreased significantly(p<0.05).In the colon,the content of Acetic acid,Isobutyric acid and Valeric acid increased significantly(p<0.05),while the Isovaleric acid content increased,the tea Polyphenols in the high-dose group and the low-dose group increased Isovaleric acid content significantly(p<0.05).The content of Propionic acid and Butyric acid was decreased significantly(p<0.05);In the large intestine,the content of Acetic acid,Isobutyric acid,Valeric acid and Isovaleric acid was increased significantly(p<0.05),and the Propionic acid and Butyric acid content were decreased significantly(p<0.05).Conclusion:(1)Tea Polyphenols can increase the diversity of intestinal microflora,reduce the diversity of bacteria in the cecum,colon and large intestine,promote the growth of beneficial bacteria,inhibit the growth of harmful bacteria,and regulate the composition of intestinal flora.(2)Tea Polyphenols can increase Trypsin activity in the intestine,decrease the activity of Amylase and Lipase in the cecum,and increase the activity of Lipase in small intestine.Tea Polyphenols decreased Cellulase activity in the small intestine,cecum and large intestine,increased the activity of the whole intestinal Xylanase,and had no significant effect on the Cellulase activity in the colon.(3)Tea Polyphenols can increase the content of short-chain fatty acids in the small intestine,decrease the content of Propionic acid and Butyrate in the cecum,colon and large intestine,and increase the content of Acetic acid,Butyric acid,Valeric acid and Isovaleric acid.The experimental results show that,in vivo,tea Polyphenols can affect the intestinal flora,intestinal enzyme activity and short-chain fatty acid to regulate the body,providing a theoretical basis for the extensive use of tea Polyphenols.