Effects Of Glucocorticoid On The Expressions Of C-Jun And C-Fos In T Cells Of Children With Idiopathic Nephrotic Syndrome In Vitro

BackgroundIdiopathic nephrotic syndrome(INS)is the most common glomerular disease in childhood,accounting for about 90% of NS in childhood.The pathogenesis is still unclear.The study suggests that it is associated with dysfunction of T cells.Glucocorticoid(GC)is the first choice for the treatment of INS in children.Most children are sensitive to glucocorticoids,but 20% of them are still resistant to hormone,and the disease can not be cured,and eventually develop to end-stage renal disease.Therefore,exploring the mechanism of glucocorticoid resistance has been a hot research topic.In recent years,some studies have shown that the disorder of transcription factor activator protein-1(AP-1)activity may be related to the pathogenesis of hormone resistance.In the course of GC treatment of INS and hormone resistance,the gene expression of T cells will change accordingly.Previous studies showed that the expression of c-Jun and c-Fos in peripheral blood T cells of children with INS increased significantly after GC treatment.Therefore,T cells from peripheral blood of children with INS were cultured in vitro,and different concentrations of hydroprednisone were added in turn to further verify the effect of GC on the expression of c-Jun and c-Fos in T cells,and to explore its clinical significance,in order to find out the molecular mechanism of glucocorticoid resistance.ObjectiveTo detect the expression of c-Jun and c-Fos in peripheral blood T cells of children with INS before and after the intervention of different concentrations of hydroprednisone,and to explore the effect of hormones on the expression of c-Jun and c-Fos and its clinical significance.MethodsPeripheral blood T cells of 48 children with INS were cultured in vitro.They were divided into control group,low concentration group and high concentration group.Different concentrations of hydroprednisone were added to make the concentration of hydroprednisone 10mM、20mM,The control group was not treated with hydroprednisone.The T cells cultured in vitro were collected,and the expressions of c-Jun and c-Fos mRNA and protein in T cells before and after hydroprednisone intervention were detected by qPCR and Western Blotting.ResultsThe results of qPCR and Western Blotting showed that the expression levels of c-Jun and c-Fos in T cells of children with INS cultured in vitro were as follows: high concentration group > low concentration group > control group,high concentration group and low concentration group compared with control group,the difference was statistically significant(*P<0.05,** P<0.01),and the higher the concentration of hydroprednisone,the higher the expression of c-Jun and c-Fos.ConclusionHydroprednisone can significantly increase the expression of c-Jun and c-Fos in peripheral blood T cells of children with INS in vitro,and the concentration of hydroprednisone is dose-dependent with the expression of c-Jun,c-Fos mRNA and protein.It suggests that glucocorticoid can increase the expression of c-Jun and c-Fos in T cells of children with INS while treating children with INS,which may affect the sensitivity of INS to hormone therapy.