The Effects Of Fomes Officinals Ames Polysaccharides On The Inflammatory Response In BV-2 Microglial Cells Induced By Aβ_25-35

Objective:To investigate the inhibitory effects of Fomes Officinals Ames polysaccharides（FOPS）and Fomes Officinals Ames polysaccharides components（FOP-a）on the inflammatory response in BV-2 microglial cells induced by Aβ_25-35 in condensed state as inflammatory cell model in vitro.Methods:BV-2 microglia cells were treated with different concentrations of FOPS and FOPS-a（6.25、12.5、25、50、100、200、400μg/mL）and cell viability was tested by MTT.A harmless concentration（6.25、12.5、25μmol/L）of FOPS and FOPS-a were used for intervention on the follow-up inflammatory model.The changes of BV-2 microglia cellular morphology were observed under inverted phase contrast microscope.The levels of IL-1、IL-6、TNF-αand MCP-1 expression were measured by ELISA assay.The lactate dehydrogenase activity in BV-2 cells were measured by LDH kit assay.The expression of inflammatory IL-6、TNF-a、MCP-1、iNOS、COX-2 and NF-κB were measured by QPCR.The expression of the iNOS、COX-2、IκBαand NF-κB were analyzed by Western blot assay.Results:MTT assay demonstrated that FOPS and FOP-a had no toxic effect on cell viability exposure to the concentration was6.25²5μg/mL.Compared with model group,the（6.25、12.5、25μg/mL）FOPS and FOP-a could inhibit the morphological changes of BV-2 cell induced by Aβ_25-35.The gene expression of IL-6、TNF-a、MCP-1、iNOS、COX-2 and NF-κB in BV-2 cells and the production of pro-inflammatory cytokines IL-1、IL-6、TNF-α、MCP-1 and LDH activity expression in Aβ_25-35 stimulation groups were significantly attenuated by FOPS and FOPS-a（p<0.05）.The increasing expression of iNOS and COX-2 and nuclear translocation of NF-κB p65 were suppressed by FOPS and FOP-a as well as the decreased expression of IκBαincytoplasm was attenuated by FOPS and FOP-a（p<0.05）.Conclusion:1.Aβ_25-35 could activate BV-2 cell and up-regulate the expression levels of inflammation factors such as IL-1,IL-6,TNF-αand MCP-1.2.FOPS and FOP-a could inhibit the expression of IL-1,IL-6,TNF-αand MCP-1 induced by Aβ_25-35 activated BV-2cell.2.FOPS and FOP-a could inhibit the expression of IL-1,IL-6,TNF-α,MCP-1induced by Aβ_25-35 activated BV-2 cell.3.FOPS and FOP-a could inhibit the inflammatory response of BV-2 cell induced by Aβ_25-35 by inhibiting the expression of NF-κBp65/IκBαin NF-κB signaling pathway.4.FOPS and FOP-a inhibit the expression of COX-2 and iNOS by inhibit NF-κB signaling pathway and further inhibit the inflammatory response of microglial induced by Aβ_25-35.