| Traditional Chinese medicine has its unique advantages in the treatment of cancer.Dahuangzhechong pills(DHZCP)are the classic prescription for treating tumors.However,due to the complexity of the traditional Chinese medicine,the pharmacodynamic substances in DHZCP are not clear,which directly affects the in-depth study of its mechanism of action.There is no doubt that it is clear that the anti-tumor effect of DHZCP is further Prerequisites for conducting pharmacological mechanisms.This paper mainly studies the identification of unknown components,the levels of cells to comprehensively investigate the anti-tumor pharmacodynamics,and the pharmacokinetic study of main components in vivo of DHZCP,in order to further explain the science of pharmacodynamics of DHZCP intervention in tumors Connotation.HPLC was used to study the fingerprint of DHZCP,and the fingerprint of DHZCP was established.The preliminary quality standard control of the commercially available DHZCP was carried out.On the basis of objectively characterizing the overall chemical information of DHZCP,through similarity analysis,principal component analysis and cluster analysis,as well as peak-to-peak ratio statistics of variance and shared peaks,and using chemical reference materials,their characteristic components Conduct a qualitative analysis.At the same time,in order to verify these ingredients and more comprehensively obtain the main components of Rhubarb Locust Pills,UPLC-Q-TOF-MS/MS technology and Peakview analysis software were used to identify 137 chemical components in DHZCP.High composition,and the ingredients of this ingredient have been tainted,including 15 kinds of rhubarb,20 kinds of rhubarb,11 kinds of Scutellaria,13 kinds of peach kernel and bitter almond,16 kinds of white peony,4 kinds of licorice,58 kinds of animal drugs.Combined with HPLC fingerprint results,20 kinds of characteristic components(Hypoxanthine,Allantoin,Gallic acid,Amygdalin,2,5-dihydroxyb enzoic acid,Paeoni florin,Verbascoside,4-Hydroxyphenylacetic acid,Liquiritigenin,Baicalin,Naringenin,Paeonol,Baicalein,Wogonin,Aloeemodin,glycyrrhizic acid,Rhein,Emodin,Chrysophanol,and Physcion)were determined by HPLC-UV.The results showed that the content of gallic acid in Baiji was the highest(2445.10±755.97)μg/g,followed by baicalin(2252.9±547.00)pg/g,amygdalin(2164.10±694.51)μg/g and verbascoside(1795.83±604.25)μg/g and other components.The content of various anthraquinones in’Junyao’Rhubarb is slightly different,with the highest level of chrysophanol,followed by lower levels of emodin,rhein,aloe-emodin and emodin;the higher content of hypoxanthine in animal drugs;The lower ones are components such as baicalein and gentisic acid,which are less than 150μg/g.Since the drug plays an effective role in the blood component,we have studied the blood components of DHZCP.The results show that not all medicinal ingredients can enter the blood.In the semi-quantitative analysis of the blood components,we found that The content of the ingredients in the square has a higher proportion in the blood components,which may be related to the conversion or metabolism of the substance.On this basis,through cell experiments,the effects of the main components of DHZCP on the viability of drug-resistant liver cancer cells SMMC-7721/DOX were studied,in order to obtain as much as possible the material basis related to the energy metabolism of rhubarb mites.We found hypoxanthine,Five components,such as rhein,emodin,aloe-emodin and wogonin,have strong inhibitory effects on the activity of drug-resistant liver cancer cells,which may be the main pharmacodynamic substance of rhubarb mites pills directly inhibiting SMMC-7721/DOX activity.Finally,we selected these blood components as much as possible to study thepharmacokinetic behavior of DHZCP in rats.The results showed that in vivo bioavailability of the active ingredients increased to varying degrees.In summary,this paper objectively characterizes the chemical information of anti-tumor active ingredients of DHZCP through three different levels,and combines cell experiments and pharmacokinetic studies to explore the material basis of energy metabolism in drug-resistant liver cancer cells. |
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