| Objective Using non-targeted metabolomics technique,we detected and analyzed the follicular fluid of infertile patients with normal female and hypogonadism,and explore the biomarkers related to infertility with poor ovarian response Kidney yin deficiency syndrome,from the Perspective of Follicular Fluid Metabonomics study the substance basis of syndromes of Infertility with poor ovarian response Kidney yin deficiency syndrome.Methods 30 cases of IVF-ET / ICSI-ET patients with low ovarian response and syndrome differentiation of kidney yin deficiency were selected.Another 49 cases of control group were IVF-ET / ICSI-ET only because of male factor.Agent program,Gn given from the third day of menstruation,when the dominant follicle reached 12 mm,add Cassia Kay to HCG day,two follicular fluid was collected from patients taking the day.The age,BMI,duration of infertility,b FSH and b AFC were observed in both groups.The levels of Gn and sex hormones during ovulation induction were compared.Oviparization,fertilization and embryo development were compared between the two groups.Complete data were collected using SCIEX High-Resolution Triple TOF? 5600+ and non-targeted metabolomics studies were performed on both follicular fluids using a secondary database of robust endogenous metabolites and a proprietary metabolomics analysis software,XCMSplus,to identify potential Of biomarkers.Results(1)There was no significant difference between the POR group and the control group in age,BMI and duration of infertility(P> 0.05).The levels of b AFC and b FSH in the POR group were 4.87 ± 2.03 and 13.5797 ± 3.609 m IU / ml,respectively,(17.45 ± 3.87,6.4849 ± 1.203 m IU / ml,respectively).The difference between the two groups was statistically significant(P <0.001).There was no significant difference in Gn days between the two groups during ovulation induction,but the Gn dosage POR group was significantly higher than the control group,the difference was statistically significant(P <0.001);HCG E2,HCG 14 mm above the number of oocytes,the number of oocytes,M Ⅱoocytes,available embryos and excellent embryos POR group was significantly lower(P <0.05).There was no significant difference between the two groups in the rate of oocytes-harvesting,MⅡ-oocyte rate,IVF fertilization rate,ICSI fertilization rate,2PN cleavage rate,available embryo rate and excellent embryo rate > 0.05),but the POR group was lower than the normal group.(2)Based on metabonomics analysis,30 different metabolites were identified and could be potential biomarkers.This metabolome group is involved in tyrosine metabolism,cholesterol biosynthesis,fatty acid metabolism,lipid metabolism,dynorphin metabolism,retinoic acid metabolism,pyrimidine metabolism,secondary metabolite synthesis,vitamin D3 metabolism and vitamin E metabolism.Metabolites in which upregulation include L-Phenylalanine,Proline,Tryptophan,1-hydroxy-2-oxypropyl tetrahydropterin,Lyso PC(16: 4),Lyso PC(18: 2),Lyso PC(18: 2),Magnesium salicylate,Uridine 5’-diphosphate,(18: 1);down-regulated metabolites include 6-Oxohexanoate,Linoleate,Oleic acid,Hexadecanal,Arachidonate,Acid,DHA,13,14-Dihydroretinol,Lithocholate,7-Dehydrodesmosterol,stearoylcarnitine,25-Hydroxyvitamin D3,27-Hydroxycholestero,3alpha,7alpha,12alpha-Trihydroxy-5beta-cholestan-26-al,13’-hydroxy-alpha-tocopherol,Dy norphin A,Lyso PC(16: 1),Lyso PC(16: 0),Lyso PC(18: 0),Lyso PC(20: 5),Lyso PC(20: 4),Lyso PC(20: 3).Conclusions(1)Compared with the normal population,the patients with low yin deficiency kidney yin deficiency syndrome have significantly lower ovarian reserve than the normal female and ovary response than the normal female,and both are lower than the normal female in terms of oocytes’ number and quality;(2)There was a significant difference in follicular fluid metabolites between patients with and without kidney yin deficiency syndrome,which could serve as potential biomarkers of infertility with kidney yin deficiency syndrome;... |
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