Comparative Study On The Determination Of Saponins And Carbohydrates In Chinese Medicinal Materials By Evaporative Light Scattering And Ultraviolet Methods

OBJECTIVE:To compare the advantages and disadvantages of two kinds of detectors,UV and ELSD,in the detection of saponins and carbohydrates in Chinese medicinal materials.METHOD:Firstly?Using orthogonal test method to optimize the conditions of the evaporative light scattering detector.Secondly,open the two detectors at the same time for HPLC-UV-ELSD detection to determine the saponins in Panax notoginseng and Radix Bupleuri and carbohydrates in Radix Glehniae.Establish of fingerprints of Radix Bupleuri and Panax notoginseng,and the chromatographic fingerprint evaluation system was developed by the State Pharmacopoeia Commission Study Edition(2004A)to evaluate the system.Finally,ELSD was used to determine the content of sucrose in different sources and batches of Radix Glehniae,and verify the feasibility of ELSD for the determination the content of carbohydrates,especially sucroseRESULTS:The optimized conditions of the detection of evaporative light scattering detector were:(1)the detection conditions of Panax notoginseng were:the gas pressure was 20psi,the spray heating level was 90%,and the drift tube temperature was 80°C.(2)the test conditions of Radix Bupleuri were:the gas pressure was 20psi,the spray heating level was 90%,and the drift tube temperature was 90°C.(3)the detection conditions of Radix Glehniae were:the gas pressure was 20psi,the spray heating level was 100%,and the drift tube temperature was 60℃.It is found that the precision of the two detectors is not very different by comparison.There were no significant differences in the fingerprints of Panax notoginseng and Radix Bupleuri with ELSD and UV detectors,but the detection results by evaporative light scattering method shows free baseline drift,less peak number,and mainly for the chromatographic peak of saponins.In addition,ELSD shows detection precision,stability and repeatability for detection of carbohydrate compounds in Radix Glehniae,and can be used for the quantitative research.CONCLUSION:The evaporative light scattering detector has a certain advantage over the UV detector in the detection of saponins and carbohydrates in traditional Chinese medicine.