Quality Standard And Main Pharmacodynamics Of Compound Jiang Tang NO.3 Granule

Objective: To study the Quality standard and main pharmacodynamics of compound JiangTang NO.3 granule.Methods:(1)The compound hypoglycemic grain quality standard NO.3: using thin layer chromatography(TLC)identification of compound hypoglycemic 3 particles in radix pseudostellariae,yam,tuckahoe,atractylodes,radix puerariae,the active components of traditional Chinese medicine(TCM),high performance liquid chromatography(HPLC)method for preparation of paeoniflorin,puerarin,determination of content of hesperidin and methodology.(2)To study the toxicity of compound JiangTang NO.3 granules.(3)To study the main pharmacodynamics of compound JiangTang NO.3 granule in the treatment of diabetes.Results: TLC was used for the identification of radix pseudostellariae,poria cocos,radix puerariae,atractylodes macrocephala and Chinese yam.The content of paeoniflorin,puerarin and hesperidin was determined by HPLC.Paeoniflorin and puerarin used acetonitrile-0.1% phosphoric acid(15: :85)as mobile phase.Column temperature:(30±5)℃,the flow rate: 1 mL/min;Detection wavelength: 230 nm,injection volume: 10 uL.Hesperidin took methanol-acetic acid-water(35:6:61)as mobile phase.Column temperature:(30±5)℃,the flow rate: 1 mL/min;Detection wavelength: 283 nm,injection volume: 10 uL.The high dose group of compound JiangTang NO.3 granule can effectively treat diabetes and reduce the indexes of diabetic mice.Conclusion: the production technology of compound JianTang NO.3 granule is reasonable and feasible.The established quality standard has good accuracy and reproducibility,and can effectively control the quality of the preparation.The bloodglucose,TNF-αand NF-kB levels of diabetic mice were decreased.The high dose group of compound JiangTang NO.3 granule could effectively reduce the blood glucose of diabetic mice,increase serum insulin(Ins),decrease insulin sensitivity index(HOMA-IR)and reduce glycosylated hemoglobin(HbAlc).The mRNA levels of AKT,GSK-3β,FOX01,PEPCK and G6 P in liver tissues increased.The expressions of GSK-3β and PCK2 protein in liver tissue were decreased.