Effects Of Paternal Obesity On Mitochondria Damage Of Testicular In Male Offspring Rats

Objective In recent years,it was suggested that the sperm representative type was damaged by paternal obesity through sperm cells.In this study,we emphasized the importance of the effect of paternal obesity on the growth and reproductive function for male offspring rats.And discussed the damage of mitochondria in testicular tissues.So as to provide theoretical basis for preventing the occurrence and development of obesity and studying the cross-generational genetic pathogenesis.Methods In this study,we selected fifteen Wistar male rats aged four weeks,which were fed in a single cage for 3 days and induced by a high-fat diet for 2 weeks.Those rats ranged according to the weight gain level,five rats were selected as the paternal obesity group(FODIO)and another five rats were selected as the normal control group(FOCON),then these rats were fed with diet for 10 weeks.In addition,sixty clean Wistar female rats aged seven weeks were fed in a single cage for 3 days.After 2 weeks high-fat diet,those female rats were ordered according to the weight gain level,and those middle third were selected as normal maternal group.After two weeks of feeding with basic diet,they were mated with two groups of male rats.Twenty normal maternal rats were mated with two groups of male rats at the ratio of 2:1 per night.The pudendal suppositories were examined in the next morning,and the pregnant rats were fed separately until delivery.After 21 days,fifteen male offspring rats were selected from weaning obese offspring group(F1HFD)and control offspring group(F1CON),respectively.The offspring of the two groups were fed with basic diet for 7 weeks,and were killed to collected serum and organ tissues.Finally,measuring the level of serum glucose,total cholesterol(T-CHO),triglyceride(TG),high density lipoprotein(HDL-C)and low density lipoprotein(LDL-C).Besides,sperm quality,reproductive hormone and oxidative damage in testicular tissue were measured and mitochondrial membrane potential(MMP)and Complex Ⅰ,Ⅱ,Ⅲ,Ⅳand V were also tested.Results Compared with the paternal control group,the weight of father offspring increased significantly,and the levels of TG,HDL-C and insulin were significantly different,but there was no significant change in blood glucose between the two groups.At the same time,the SCA sperm quality analyzer showed that the forward motility rate of sperm decreased,the rate of sperm malformation increased,but there was no significant difference in sperm density;the levels of testosterone(T),luteinizing hormone(LH),glutathione peroxidase(GSH-Px)in testicular tissue decreased,and the level of malondialdehyde(MDA)active oxygen(ROS)decreased.The level of Complexes Ⅱ and Ⅲ were significantly decreased,while the levels of Complexes Ⅰ,Ⅳ,Ⅴ and MMP were not significantly difference.Conclusion Paternal obesity can affect the reproductive function of offspring and induce mitochondria damage in testicular tissue of offspring.At the same time,paternal obesity can induce disorders of glucose and lipid metabolism in offspring,suggesting that paternal obesity increases the risk of obesity and metabolic syndrome in offspring.