The Effect Of DDB2 Gene On Acne Hypertropgic Scar And TGF-β/Smads Signal Pathway

Objective: Acne vulgaris is a common kind chronic inflammatory disease of dermatological department.According to international meliorating classification,acne vulgaris consists of I,II,III and IV levels(mild,moderate and severe acne vulgaris).The primary lesions of severe acne vulgaris are cyst and nodule,frequently leading to scar.Lesions above mentioned brings damage on appearance,and then followed by negative emotion.A growing number of researches have found that genetic factors play an important role in acne hypertrophic scar.Therefore,this study planned to investigate the effect of DDB2 gene on acne hypertropgic scar and TGF-β/Smads signal pathway.Methods: 1.Constructing 2 different kinds of lentivirus vectors.Stable clones expressing over DDB2(group pCDH-GFP-DDB2)and suppressing DDB2(group DDB2-shRNA)were used.Then the lentivirus particles mentioned above were infected into human FB cells.Besides the 2 groups mentioned before,3 more ones were involed,including group pCDH-GFP,group scrambled-shRNA and group Blank.2.The growth ability of human FB in different groups was detected by MTT,while the death ability was detected by flow cytometry.3.Western blot was performed to examine the expression level of TGF-β/Smads.Results:1.Western blot results: Compared to group pCDH-GFP,group pCDH-GFP-DDB2 showed significant up-regulatingexpression level of DDB2 protein;compared to group scrambled-shRNA,group DDB2-shRNA showed down-regulating expression level of DDB2 protein.The overexpression lentivirus vector,the interference lentivirus vector and transfected cell lines were constructed.2.Compared with group Blank,group pCDH-GFP-DDB2 showed higher growth ability(P<0.05)and group DDB2-shRNA showed lower growth ability(P<0.05).3.By flow cytometry,group pCDH-GFP-DDB2 showed lower death ability than group Blank and group pCDH-GFP(P<0.05),and group DDB2-shRNA showed higher death ability than group Blank and group scrambled-shRNA(P<0.05).4.Western blot showed lower expression level of TGF-β/Smads in group pCDH-GFP-DDB2 and higher one in group DDB2-shRNA.Conclusions: 1.DDB2 gene efficiently inhibits proliferation of human FB cells.2.DDB2 gene efficiently induces apoptosis of human FB cells.3.TGF-β/Smads signal pathway negatively correlates to DDB2 gene.4.It is speculated that DDB2 gene participates in the pathogenic process of scar by specifically targeting TGF-β/Smads signal pathway and regulating proliferation and apoptosis of human FB cells.