Study On The Patterns Of Compatibility And Treatment Features Of Prescriptions Containing Bupleri Radix-Scutellariae Radix During Past Dynasties

The combination of Bupleuri Radix-Scutellariae Radix was first recorded in Treatise on Febrile Diseases,it is one of the most important part of Bupleurum analogous decoctions-there are lots of prescriptions containing Bupleuri Radix through ages.These prescriptions have been widely used in the treatment of exogenous diseases-internal wounds.While there exists very little research has focused on the compatibility rule-pattern-treatment features of these prescriptions.Therefore,this study collects-analyses sort out the prescriptions of Bupleri Radix-Scutellariae Radix recorded in historical medical literature,-collect statistics of the compatibility proportions of Bupleri Radix-Scutellariae Radix,as well as composed medicines,diseases of the main treatment,symptoms-tongue veins in these prescriptions,-use data mining method to make a preliminary investigation on their compatibility rules-pattern-treatment features under the guidance of TCM theory.Combining with experimental research,the relationship between compatibility of Bupleri Radix-Scutellariae Radix-anti-depression effect were preliminarily investigated.From the antidepressant dimension to clarify the scientific connotation of the compatibility of Bupleri Radix-Scutellariae Radix,for the purpose of providing reference for clinical medication.This paper is mainly divided into two parts.The first part is data mining.Using data mining method,966 prescriptions of Bupleri Radix-Scutellariae Radix were collected-st-ardized compatibility proportion of Bupleri-Radix Scutellariae,composed medicines as well as diseases,symptoms-tongue veins in prescriptions were sorted out-recorded in the Excel database,then SPSS Statistics 22.0 was used to analyze the Excel database data.SPSS Modeler 18.0 was used for association analysis of data.-Gephi0.9.2 was used for complex network analysis of the prescriptions.Date mining results showed that:Part 1:Study on study on the patterns of compatibility and treatment features of prescriptions containing Bupleri Radix-Scutellariae Radix Bupleri Radix-Scutellariae Radix by data mining.Method:1202 chaiqin prescription were collected,and an Excel database was established.SPSS Statistics 22.0 was used to conduct frequency Statistics on drugs,syndromes,diseases and symptoms in Excel database data.SPSS Modeler 18.0 was used for association analysis.And use Gephi 0.9.2 for complex network analysis.1.The compatibility rule of Bupleuri Radix-Scutellariae Radix:The major categories of drugs containing Chaiqi prescription are mainly heat-clearing drugs,tonic drugs,solution-solving drugs,phlegm-removing drugs,qi drugs,the compatibility ratio of bupleur-scutellaria in the han dynasty was mainly 8:3,which is the main ratio in zhongjing’s formulas.Subsequently,the compatibility ratio of Bupleuri Radix-Scutellariae Radix evolved with the change of times.In the compatibility relationship between the two herbs,the number of prescriptions with a higher dosage of bupleur-scutellaria was gradually reduced,while the number of prescriptions with a lower dosage of bupleur-scutellaria was gradually increased.From tang-song dynasties to now,the number of prescriptions with the same dosage has always been the first place.2.Compatibility of the prescriptions containing Bupleri Radix-Scutellariae Radix:The commonly used medicine types of these prescriptions include antifebirile,tonic medicines,exterior-relieving medicines,expectorant medicines,Qi-regulating medicines.The commonly used small categories medicines are wind-cold-effusing medicine,heat-clearing and fire-purging medicine,blood-repienishing medicine,qi-invigorating medicine,heat-clearing and blood-cooling medicine,wind-heat-effusing medicinal,etc.Commonly used herbs are ginseng.Pinellia,Angelica,Angelica,Scorpion,Chuanxiong,Tangerine,Poria,Rhubarb,Li Hemp,clam shell,Zhi mu,platy codon and so on.The two-medicine combination compatible with Bupleri Radix-Radix Scutellariae frequently are ginseng-Pinellia,white peony-angelica,white peony-Chuanxiong,Pinellia-tangerine peel,cockroach-wind,angelica-rehmannia,medlar-gentian,Chuanxiong-white peony,rhubarb-mirabilite,windproof-schizonepeta,ginseng-atractylodes,alfalfa-atractylodes,ginseng-armor and so on.The three-medicine combination compatible with Bupleri Radix-Radix Scutellariae frequently are Chuanxiong-white peony-angelica,angelica-white peony-ginseng,atractylodes-cockroach-ginseng,angelica-Chuanxiong-ginseng,angelica-poria cocos-white peony root and so on.Its medicine composition could be divided into four parts,including ①the composition prescriptions of Bupleri Radix-Scutellariae Radix that composed of Pinellia,as the core;③the composition prescriptions of Bupleri Radix-Radix Scutellariae that composed of notopterygium root,the root of fangfeng as the core;②the composition prescriptions of Bupleri Radix-Scutellariae Radix that composed of Angelica sinensis and Paeoniae alba as the core;④the composition prescriptions that composed of Gardenia as the core.3.The deseases,syndrome elements and symptoms:(1)Among the 145 diseases treated by these prescriptions,medical diseases ranked first,followed by surgical diseases and gynecological diseases,and there were few diseases in otolaryngology.Specific diseases include typhoid fever,malaria,fatigue,cold,irregular menstruation,plague,bludgeoning,jaundice,etc.(2)The main therapeutic syndromes include both deficiency syndromes and sufficiency syndromes,with deficiency syndromes as the main therapeutic syndromes.In specific deficiency syndromes,hot(fire)be in first place,wind is next,poison,cold again,other disease is also visible.When it comes to the sufficiency syndromes,blood deficiency first,qi deficiency second,Yin deficiency third.The disease position syndrome has the zang-fu disease position and the six-channel disease position.Among the zang-fu disease sites,liver and shao-yang gallbladder were the first,followed by stomach,heart,spleen and lung.Among the lesions of the six meridians,shao-yang was the first,yang-ming and sun were the second.Liver,gallbladder,spleen and stomach were the most common sites.(3)There were a total of 260 symptoms including,of which 60 were high-frequency symptoms,with fever,headache,cold and heat exchanges the highest,followed by loss of appetite,cough,thirst,upset,strong fever,etc.High-frequency symptoms can be classified into 15 categories,among all these ctaegories fever-related symptoms are the most commonly treated,followed by symptoms in the chest and emotional symptoms in the third.The common pulse conditions are pulse string,pulse number,pulse flood,pulse float,etc.Common tongue for the tongue stem is yellow moss.The results of pattern-treatment feature analysis showed that the most common symptoms were:(1)the two symptoms:fever-headache,headache-thirst,etc.The subjects with high confidence were headache-cold aversion,chest fullness-bulging,anorexia-wasting,fever-pulse number,pulse string-pulse number,etc.The number of pulse-pulse string,headache-aversion to cold,headache-strong heat were all high in support and confidence.(2)Three symptoms:high degree of support for headache-cold-fever,headache-vomiting-cold and heat exchanges;The subjects with high confidence were headache-strong heat-aversion to cold,pulse number-pulse string-cold and heat exchange,strong heat-upset-headache,vomiting-cold and heat exchange-headache,etc.Those with high support and confidence were headache-cold aversion-fever4.Relationship between syndrome characteristics and herbs of four common diseases(1)Typhoid fever:the core network of typhoid diseases can be divided into three communitiesCommunity 1 showed common symptoms for cold and heat exchanges,vomiting,chest full,is a common symptom of shao-yang syndromeCommonly used drugs for ginseng,pinellia ternata,orange peel,ginger,immature bitter orangeCommunity 2 showed that:Common symptoms were fever,strong heat,cold and heat exchanges,chest full,upset,vomiting,stool secret knot,which were the mainly symptoms of shao-yang and yang-ming.Commonly used drugs include:main drugs containing pinellia ternata,gardenia,ginger,angelica,immature bitter orange,rhubarb,mirabilite,gypsum,red peony;secondary drugs are anemarrhena,rhinoceros horn,radix ophiopogonis,radix puerariae,radix scrophulariae;Community 3 showed that:Common symptoms were fever,headache,aversion to cold,strong heat,cough,chest full,vomiting,which were the commonly symptoms of tai-yang shao-yang.Commonly used drugs include:main drugs were fang feng,qiang live,gardenia,orange peel,chuanxiong,orange root,wormwood,ginger,pinellia ternata;secondary drugs containing kudzu root,raw land,almond,cohosh(2)The results of the core network of malaria showed that the common symptoms include:the main symptoms were cold and heat exchange,and the secondary symptoms were fever,diarrhea,headache and coughCommonly used drugs include:main drugs that contained pinellia ternata,orange peel,ginseng,atractylodes rhizome,grass fruit;secondary drugs which contained poria cocos,atractylodes macrocephala,green bark,magnolia bark,etc.(3)The results of core network of deficiency and fatigue showed that the common symptoms included:wasting,loss of appetite,cough,bone steaming and heat,and upset;secondary symptoms include limb pain,fever,night sweats,etcCommonly used drugs include:main drugs included ginseng,angelica,turtle shell,radix ophiopogonis,astragalus,radix coleae,radix paeoniae alba,radix platycodonis;secondary drugs containing anemarrhena rhizoma,poria cocos,atractylodes macrocephala,radix pinelliae,pinellia tuber,rhizoma Chen PI,rhizoma chuanxiong,gentiana macrophylla,etc(4)Cold core network results common symptoms include:main symptoms fever,headache,loss of appetite,cold and heat exchange,cold aversion,vomiting,diarrhea,abdominal fullness.cough;secondary symptoms:abdominal pain,chest pain,joint pain,pain in the mouth,sweating,etc.Commonly used drugs for qiang-huo,fang-feng,chuan-xiong,white peony root,pinellia terebinata,orange peel,orange stem,fructus aurantii,mint,poria.Part2:Experiment research.Method:Uniform design U7(72)table was choosen to divide Bupleuri Radix-Scutellariae Radix Chronic-the dosage of the herbs kept in with bounds of the Chinese pharmacopoeia.Unpredictable mild stress(CUMS)were taken for modeling-the antidepressant effects were observed by behavioral tests such as sucrose preference-forced swimming test.Then the results of behavior tests were analyzed by multivariate regression analysis to obtain the best compatibility ratio.Confirmatory experiment,The optimal dosage ratio of 1:1 was compared with the dosage ratio of 8:3 and the opposite ratio of 3:8 in"treatise on febrile disease".Meanwhile,the effects of bupleur-scutellaria on the CREB/BDNF signaling pathway and the morphology and number of neisseria corpuscle in the hippocampus CA3 region of mice were observed to analyze and explore the possible anti-depression mechanism of bupleur-scutellariaResults:The results of uniform design study showed that compared with the blank group,the sucrose preference rate and the number of crossing of model group were significantly decreased,the immobility time of tail suspension test and forced swimming test were significant increased(P<0.01).While treating with the decoctions of Bupleuri Radix-Scutellariae Radix Compared with the model group,the sucrose preference rat and the crossing number of the Bupleri Radix-Scutellariae Radix 1:1 group、the Bupleri Radix-Scutellariae Radix 8:3 group were increased(P<0.01 or P<0.05),the immobility time of tail suspension was decreased(P<0.01 or P<0.05),the number of crossing was also decreased in the Bupleri Radix-ScutellariaeRadix 1:1 group(P<0.01).The results of behavioral tests were analyzed by regression analysis,which showed that the best compatibility ratio of Bupleura Radix-Scutellaria Radix was 1:1.Confirmatory experiment showed that the compatibility of Bupleuri Radix-scutellariae Radix could alleviate the depressant situation of mice(P<0.05,P<0.01),-the compatibility of radix bupleuri-scutellariae radix(1:1)group in the forced swimming test、tail suspension test is better than that of the rest groups(P<0.05,P<0.01),-this compatibility was also superior to the compatibility of Bupleuri Radix-scutellariae Radix(3:8)in sugar water preference test、open field test(P<0.05).Western Blotting results showed that comparing with the blank group,the phosphorylation levels of CREB the expression of BDNF,the number of positive Nissl bodies were decreased in model group(P<0.01).After treating with intragastric administration the phosphorylation levels of CREB,the expression of BDNF,as well as the number of positive Nissl bodies were increased(P<0.01 or P<0.05),Bupleri Radix-Scutellariae Radix(1:1)compatibility is superior to the other two compatibilities.The possible antidepressant of this compatibility may connect with the effect of protecting hippocampal neurons by promoting the phosphorylation level of CREB and the expression of BDNF in hippocampal protecting Nissl bodies in the brain.The results of this experiment showed that Bupleuri Radix-Scutellariae Radix could protect hippocampal neurons by promoting the expression of phosphorylated CREB and BDNF.The optimal dosage ratio was 1:1.