| Objective:To investigate the distribution of single nucleotide polymorphisms of VDR gene and ELOVL2 gene in Han population in a certain area of Anhui Province,and to investigate the nutritional intake of vitamin D and polyunsaturated fatty acids in the study population,and analyze 5 singles.The relationship between nucleotide polymorphisms and the risk of tuberculosis in Han population in a certain area of Anhui Province.Methods:This study used a case-control design method to collect 326 patients with pulmonary tuberculosis who met the diagnostie criteria(GB15985-1995)from the East Lung and West Lung Respiratory Department of Anhui Huainan Dongfang Group Hospital Cancer Hospital,and in accordance with the principle of frequency matching,in Huainan The People's Hospital of the People's Hospital of the West Hospital collected 330 healthy controls that matched the age,gender,and ethnicity;Two candidate sites of VDR gene,rs3847987 and rs739837,and three candidate sites of ELOVL2,rs3798719,rs1570069 and rs2236212,were selected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)and Sanger sequencing.Analytical methods were used for genotyping.Data were analyzed using Excel2010 and SPSS20.0 software.The general situation(age,gender)between the ease group and the control group was eompared by chi-square test ?2 and t test.The experimental study object Hardy was analyzed by ?2 test.-Weinberg genetic balance,using Generalized Multifactor Dimensionality Reduction(GMDR)to analyze the interaction between five SNP loci,using online software SHEsis software to analyze the SNP between VDR gene and ELOVL2 gene Chain imbalance.Genotype,allele frequency,dominant,recessive,superdominant and homozygous models were constructed to analyze the distribution of the five SNP loci selected above between the case group and the control group.The data was entered using EpiData 3.1 software,and the data entered were analyzed in combination with SPSS 20.0 statistical software.Results:The average age of tuberculosis patients was 55.3±14.7 years old,including 232 males(71.2%)and 94 female patients(28.8%).The average age of healthy controls was 57.6±13.9 years,including 225 males(68.2%).There were 105 female patients(31.8%);there was no significant difference between the case group and the control group in gender(?2=0.691,P=0.406)and age(?2=2.936,P=0.087).The results of analysis of different genetic models showed that the ER37LL19 polymorphism of ELOVL2 gene in the case group and the control group was statistically significant in the overdominant model(?2=4.784,OR=1.414;95%CI:1.036-1.929,P=0.029).Learning differences,after stratified analysis by gender,we found that the polymorphism of ELOVL2 gene rs2236212 in male tuberculosis patients and healthy controls was in the dominant model(?2=4.192,OR=0.507;95%CI:0.262-0.981,P=0.041)The distribution was statistically different.At the same time,we also found that the ELVOL2 gene rs3798719 polymorphism in female tuberculosis patients and healthy controls was genotype(?2=6.987,P=0.030),recessive model(?2=4.936,OR=0.130;95%CI:0.016-1.063,P=0.026),homozygous model(?2=6.876,OR=0.104;95%CI:0.012-0.866,p=0.032)all have statistical differences There was no statistically significant difference between male tuberculosis patients and healthy controls.Conclusion:The single nucleotide polymorphism of rs3798719 in ELOVL2 gene may be associated with susceptibility to tuberculosis in women.The rs2236212 single nucleotide polymorphism may be associated with male tuberculosis,rs 15 70069 single nucleotide polymorphism and tuberculosis there is no correlation;the rs3 847987 and rs739837 single nucleotide polymorphism of the VDRgene may not be related factors for tuberculosis susceptibility.Figure[12]table[14]reference[53]... |
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