The Effect Of High-fructose Diet On The Course Of Fungal Keratitis

Background:Fungal keratitis is an infectious keratopathy with a very high rate of blindness caused by the infection of pathogenic fungi.In recent years,the prevalence of fungal keratitis has risen to the top of infectious keratopathy(51%to 70%).Our province is a large agricultural province,the incidence of fungal keratitis is high,and the study to reduce the blindness of fungal keratitis is of great significance.With the improvement of our living standards,sugary foods are ubiquitous,especially in children’s foods and beverages,the proportion of sugar is generally higher.A number of related studies have shown that excessive consumption of high-sugar foods affects normal glucose metabolism and causes multiple harms.Although many studies have shown that high glucose is an important cause of many diseases in the body,at the same time,many frontier studies have shown that high sugar diet may play a positive role.Therefore,this study aims to explore the effect of high-fructose diet on the course of corneal fungal infection,whether it is positive to promote repairment,or delay the unfavorable factors of healing,which is the problem that we propose and to solve.Through the experimental results,it will play a guiding role in the application and treatment of fungal keratitis infection.Object:In the present study,we aimed to observe the effect of high-fructose diet on the course of fungal keratitis.Methods:78 C57BL/6 male mice(free of eye disease)are 8~12 weeks old,SPF level.Animals were distributed into high-fructose diet group and control group randomly,each group has 78 eyes(n=78),high-fructose diet group was provided with a 10%fructose solution,control group was normal water.We measured the weight and blood glucose of two groups mice every other day.10 days later,we establish the FK model.At 24,36,48,72,96,120,168 hours,took photos using digital camera after fungal infections and assessed the clinical manifestations under slit-lamp microscope.Mice were euthanized by cervical dislocation at 24,36,48,72,96,120,168 hours after fungal inoculation,whole-mount immune-histology was conducted and we use2 PH scanning microscopy to scan and picture the middle,paracentral,limbus parts of cornea,and to analyze the mount of neutrophils and macrophages.The corneal sections were prepared for the histopathological examination and perform HE and PAS staining,to observe changes in corneal inflammation and fungal load at different time points.The protein levels indicating cytokine interleukin-1β(IL-1β)production in the cornea were quantified using enzyme-linked immunosorbent assay(ELISA)kits.Results:The clinical scores of the high-fructose diet group were significantly higher than the control group at each time point,and the differences were statistically significant.Corneal perforation rate of high-fructose diet group was 80%,control group was 43.3%,we used Chi-squared Test and difference among two groups was statistically significant(χ~2=8.531,P=0.003).The number of neutrophil in high-fructose diet group was higher than control group at 24,48,72,120,168 hours,the difference among two groups was statistically significant(P(27)0.05).At 72,96,120,168 hours,the number of macrophage in high-fructose diet group was higher than control group,the difference among two groups was statistically significant(P(27)0.05).Corneal histopathological examination showed that the inflammation reaction was more severe in the high-fructose diet group,and the corneal tissue destruction was earlier and more severe.The expression of IL-1βin high-fructose group was higher than control group at 24,48 hours,the difference among two groups was statistically significant(P(27)0.05).ConclusionHigh-fructose diet aggravates the severity of fungal keratitis infection,enhances the chemotaxis of neutrophils and macrophages,and promotes the secretion of IL-1β.