Effect Of MiR-373-3p On Proliferation And Migration Of Endometrial Carcinoma Cell Line HEC-1A

ObjectiveTo discuss the effect of miR-373-3p on proliferation and migration of human endometrial carcinoma cell line HEC-1A,and demonstrate whether LAST2 expression was regulated by miR-373-3p in endometrial carcinoma cells.This studies was aimed to investigate the influence of miR-373-3p on the biological characteristics of endometrial carcinoma and provide new ideas and approaches for potential therapeutic targets of endometrial carcinoma.MethodsmiR-373-3p mimics and inhibitor lentivirus vectors were constructed and subsequently employed to infected endometrial carcinoma cell HEC-1A,respectively.The expression level of miR-373-3p was analyzed by real-time PCR after HEC-1A cell transfection.Cell proliferation was measured by CCK-8 assay,and cell migration was detected by awound-healing assay and Transwell assay.The bioinformatics software was used to identify potential targets of miR-373-3p,and the expression of LAST2 was detected by Western Blot assay after endometrial carcinoma cell line HEC-1A transfection.ResultsDNA sequencing results demonstrated that the miR-373-3p mimics and inhibitor entiviral vector were constructed successfully.miR-373-3p mimics and inhibitor successfully up-regulated and down-regulated the expression of miR-373-3p in endometrial carcinoma cell line HEC-1A.The overexpression of miR-373-3p promotes proliferation and migration in endometrial carcinoma cell line HEC-1A.On the contrary,the expression of miR-373-3p was inhibited in endometrial carcinoma cell line HEC-1A,and cell proliferation and migration were suppressed.LATS2 could be one of the target gene of miR-373-3p by applying some bioinformatics software.Western Blot result showed that overexpression of miR-373-3p significantly inhibited the expression of LATS2 in endometrial carcinoma cell line HEC-1A,while the low expression of miR-373-3p increased the expression of LATS2 in HEC-1A cells.ConclusionmiR-373-3p play a role of oncogene in endometrial carcinoma cell line HEC-1A.The overexpression of miR-373-3p promotes the proliferation and migration in endometrial carcinoma cell line HEC-1A,while the low expression of miR-373-3p inhibits the cell proliferation and migration of HEC-1A cell.miR-373-3p may be involved in the development of endometrial carcinoma by regulating LATS2 expression and it is as a potential target for the treatment of endometrial carcinoma.