The Expression And Distribution Of AQP4 And Ngn3 In Traumatic Brain Injury

Objective:To study the expression and distribution of AQP4 and Ngn3 in the brain of traumatic brain injury,and to explore its role and regulation in traumatic brain injury.To provide a laboratory basis for the prevention and clinical treatment of brain edema after traumatic brain injury,as well as the study of the recovery of nerve function nerve and regeneration.Methods:Thirty healthy adult SD rats(male and female)were randomly divided into two groups: normal control group(n = 5)and traumatic brain injury model group(n = 25).The model group was subdivided into 5 subgroups(5 rats in each group).The rats were divided into two groups: normal control group(n = 5)and traumatic brain injury(TBI)model group(n = 25).After abdominal anesthesia with 10% chloral hydrate,a modified marmarou free-fall batter was used to hit the head of the rats.The traumatic brain injury models of rats were established at 6 h,1 d,2 d,5 d,respectively,and the model of traumatic brain injury was established at 6 h,1 d,2 d,5 d,respectively.The expression and distribution of AQP4 and Ngn3 in the brain tissue of the model group and the normal control group were observed by immunohistochemical SABC staining,and the expression and distribution of AQP4 in the brain tissue were detected by western-blot,and the expression and distribution of AQP4 in the brain tissue of the model group and the normal control group were examined by immunohistochemistry.Expression and changes of Ngn3 protein in brain tissue after traumatic brain injury.Results:AQP4 was not expressed or weakly expressed in normal rat brain tissue,and the positive reaction was mainly distributed in vascular endothelial cells and glial cells in brain tissue,while Ngn3 was mainly expressed in ependymal epithelium in normal rat brain tissue.The expression of Ngn3 was mainly expressed in ependymal epithelium in normal rat brain tissue.There was no significant change in AQP4 expression in brain tissue of TBI model group at 6 h,and there was no significant difference compared with normal control group(p > 0.05).The expression of AQP4 in the medulla,capillary endothelium and glia began to decrease on the 2nd day,and peaked again at the 5th day.The expression of AQP4 in the model group was higher than that in the normal control group on the 1st day after the first day,and the expression in the model group was higher than that in the control group.The difference was statistically significant(p < 0.05).In the model group,a small number of Ngn3-positive neurons were observed in the cerebral cortex at 6 h,and a small number of undifferentiated large granular cells were found to be Ngn3-positive in the hippocampus on the 1st day,and the number of Ngn3-positive cells in the hippocampus reached the peak on the 5th day.The expression of Ngn3 in the model group was significantly higher than that in the normal control group(p < 0.05),and the expression of VEGF in the model group was significantly higher than that in the control group(p < 0.05).Conclusion:The expression level of AQP4 in traumatic brain injury directly affects the degree of brain edema,plays an important role in the development and development of brain edema,and has important clinical significance in the prevention and target treatment of brain edema;after traumatic brain injury,the Ngn3 is transient expression in the cortical neurons,which may be related to the stress and neuronal damage of brain injury,and the undifferentiated large granular cells in the hippocampus may be neural stem cells,and the differentiation may be involved in the regeneration of the neurons after brain injury.