Multiphoton Microscopic Imaging Of Spinal Cord Injury

Spinal cord injury is a common central nervous system disease,characterized by high morbidity and disability.It is mainly caused by traffic accidents and falls,and has a profound impact on people with serious physical and mental health.The early diagnosis and evaluation of the spinal cord injury is conducive to the patients,allowing them to get timely treatment and recover from the disease as soon as possible.In this paper,Sprague-Dawley rats were chosen as experimental subjects,and multiphoton microscopy was used to image the normal and injured spinal cord tissues,demonstrating that multiphoton microscopy have the potential to be used in the diagnosis and assessment of spinal cord injury.Based on spectral and morphological information,multiphoton microscopy was used to identify and analyze the microstructure of different regions of the spinal cord including ventral horn,intermediate area,dorsal horns,ventral column,lateral column and dorsal column.In addition,multiphoton microscopy was applied to obtain high-resolution images of injured spinal cord tissues.Furthermore,the density of nerve fibers and the content of extracellular matrix in the normal and injured spinal cords were quantified base on the images.With the development of multiphoton-based endoscopic techniques and novel laser sources,we have reason to believe that this technique will open up new possibilities for the diagnosis and treatment of spinal cord diseases in the near future.