The Diagnosis Of Pituitary Adenoma And Craniopharyngioma By Use Of Multiphoton Laser Scanning Microscopy

Intracranial tumors are frequently encountered in saddle area that is near many important neural structures.They are the severe disease threatening human life and health.Pituitary adenoma is the most common tumor in saddle area which even can seriously affect patient’s life by the hormone secretion.Most of tumors in saddle area are benign,so surgery is the best option.Total tumor resection may make a recovery.But the structure of the sellar region is complex that affects the operation.If we resort to the effective imaging technique during surgery,we shall identify the boundary of tumor for total tumor resection and improve the prognosis.In recent years,multiphoton microscopy(MPM)has been used in biomedical fields widely.It has the advantages of high resolution,low damage,label-free and so on.In this study,MPM technology was used to identify the common tumor in saddle area demonstrating that this technology has the potential to diagnose the tumor in sellar region.Firstly,we visualize the calcification masses and it establishes the basis for imaging of pituitary adenoma.And then,MPM was employed to diagnose the pituitary adenoma.The results showed that this technology not only can identify normal pituitary,hyperplasia and adenoma for boundary distinction,but also can provide the different growth patterns in pituitary adenoma.At last,MPM was employed to image two subunits of craniopharyngioma for verifying the feasibility in identifying the tumor.Taken together,our results demonstrated that MPM has the potential to develop into an effective diagnostic tool for tumor in saddle area during operation when it realizes miniaturization.