The Changes And Significance Of Serum Testosterone In Diabetic Erectile Dysfunction Rats

Purpose: To investigate the changes and significance of testosterone in the development of erectile dysfunction in diabetic ratsObjective: To investigate the role of testosterone in diabetes mellitus with erectile dysfunction.Method: 3-month-old male Wistar rats(n = 18)were divided into 2 groups,the nomal contorl group(n = 9)and the diabetes mellitus with erectile dysfunction group(n = 9).The largest intracavernous pressure was mesured by CN electrostimulation.Serum levels of serum testosterone and the concentration of cGMP were measured by enzyme-linked immunosorbent assay(ELISA)respectively and the percent of corporal smooth muscle cells was detected by masson staining.Results: The largest ICP of the diabetes mellitus with erectile dysfunction group(58±11mmHg)was significantly lower than the the nomal contorl group(115±13 mmHg)(p<0.05),and serum levels of testosterone of the diabetes mellitus with erectile dysfunction group(17.45±2.54 pmol/mL)was obviously lower compared to the nomal contorl group(338.46±63.80 pmol/mL)(p<0.05).The nomal contorl group had higher the percent of corporal smooth muscle cells than the diabetes mellitus with erectile dysfunction group(p<0.05).The concentration of cGMP were significantly decreased in the diabetes group compared to the normal control group(P<0.05).Conclusion: These results suggest that testosterone could play a important role in the development of ED in STZ-induced diabetic rats.The decline of testosterone levels may play an important role via decreases of levels of serum testosterone,the percent of corporal smooth muscle cells and cavernous cyclic guanosine monophosphate in the generation of erectile dysfunction in diabetic mellitus.