| Objective:To investigate the expression of nucleotide excision repair cross-complementing gene 1(ERCC1)and thymidylate synthase(TS)in colorectal tumors,and the relationship between the two genes and the clinicopathological features of the tumor,two genes for patients with colorectal cancer The effects of treatment and prognosis after platinum and fluorouracil treatment were used to provide reference for different patients in the treatment strategy of colorectal cancer(CRC).Methods:During the two years from 2011 to 2013,84 patients who underwent radical resection of colorectal cancer at Lu’an People’s Hospital were enrolled in the study.84 patients who received m FOLFOX6 chemotherapy after surgery were included in the study.The expression of ERCC1 and TS in colorectal cancer tissues of all the patients included in the study was detected under the guidance of immunohistochemistry.The statistics and analysis were mainly assisted by SPSS 18.0 software.The expression of the two genes was analyzed.The therapeutic effects of the m FOLFOX6 regimen and the effects of patient survival and prognosis were used.Results:Among 84 patients with CRC,high expression of ERCC1 accounted for 35.7%(30/84),high expression of TS accounted for 41.7%(35/84),high expression of ERCC1 and TS accounted for 22.6%(19/84),and both ERCC1 and TS were low.Expression accounted for 34.5%(29/84).Chi-square test showed that there was a significant correlation between ERCC1,TS and ERCC1 and TS co-expression levels and DFS(P=0.001;P=0.038;P<0.001).Kaplan-Meier curves showed that patients with high expression of ERCC1 had significantly lower DFS and OS than patients with low expression of ERCC1(all P < 0.001).Univariate COX regression showed poor tumor differentiation(P=0.011),high postoperative CEA(P=0.014),and high expression of ERCC1(P<0.001)were risk factors for DFS in patients with CRC.Multivariate COX regression showed high postoperative CEA Levels(P=0.005)and high expression of ERCC1(P<0.001)were risk factors for DFS in CRC patients.Univariate and multivariate COX regression showed that ERCC1 expression was a risk factor for OS in CRC patients(P < 0.001).Patients with high TS expression were significantly lower in DFS and OS than those with low expression of ERCC1(P=0.019;P=0.084,respectively).Univariate COX regression showed poor tumor differentiation(P=0.022),high postoperative CEA(P=0.034),and high TS expression(P=0.024)were risk factors for DFS in patients with CRC.Multivariate COX regression showed high postoperative CEA Levels(P=0.036)and TS high expression(P=0.034)were risk factors for DFS in CRC patients.Multivariate COX regression showed that high TS expression was a risk factor for OS in CRC patients(P=0.053).Patients with high expression of both ERCC1 and TS had a median DFS of 18 months and a median OS of 39 months.Kaplan-Meier curve analysis showed that patients with high expression of ERCC1 and TS were significantly lower than those with low expression of ERCC1(P< 0.001).Univariate analysis showed poor tumor differentiation(P=0.034),high postoperative CEA(P=0.022),high expression of ERCC1(P=0.001),high expression of TS(P=0.024),and high expression of ERCC1 and TS(P=0.001)was a risk factor for DFS in CRC patients.High expression of ERCC1(P=0.001)and high expression of ERCC1 and TS(P<0.001)were risk factors for OS in CRC patients.Multivariate analysis showed that high postoperative CEA(P=0.038)and high expression of ERCC1 and TS(P=0.042)were risk factors for DFS in CRC patients.High expression of ERCC1 and TS was a risk factor for OS in CRC patients(P=0.046).Kaplan-Meier curve analysis showed that ERCC1 and TS expression in colon were significantly associated with colon cancer survival(P<0.001 for DFS;P=0.003 for OS),which was significantly associated with survival of rectal cancer(P=0.025 for DFS;P= for OS)0.005).Univariate and multivariate analysis showed that high expression of ERCC1 and TS were adverse factors of DFS and OS in colon cancer(P=0.001 for DFS;P=0.007 for OS),and high expression of ERCC1 and TS were rectal cancer DFS and OS.Adverse factors(P=0.055 for DFS;P=0.027 for OS).Conclusion:The expression levels of ERCC1 and TS in colorectal cancer tissues may affect the treatment outcome of patients.The co-expression of the two may be used as a kind of value in evaluating the survival and prognosis of postoperative chemotherapy in patients with colorectal cancer.The new prognostic factor plays a role in providing more practical evidence for the clinical classification of colorectal cancer after surgery for high-risk stage II and stage III adjuvant chemotherapy. |
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