Studies On The Chemical Constituents And Content Determination Of Commiphora Myrrha

This paper is divided into four parts to summarize the research work on myrrha.Firstly,In the first chapter,we give a detailed overview of myrrha,int roduce the basis and design idea of the topic.In the second chapter,the phar macological activities of myrrha were screened,and then the chemical constitue nts were studied.In the third chapter,the content determination results of myrr ha are introduced.In the last chapter,the experiment is summarized.Myrrha is a dry resin of Dingshu（Commiphora myrrha Egnl.）or Harding（Commiphora molmol Egnl.）,a plant of Commiphora of Burevaceae.It is divid ed into natural myrrha and colloidal myrrha.Myrrha has a special fragrance,a nd has a long history in China and India.It has the effect of promoting blood circulation,reducing swelling and relieving pain.positive phase column chromatography（silica gel（H,200³00 mesh）,glyc ol base）,reverse phase column chromatography(MCI,C₁₈),thin layer chromato graphy,Sephadex LH-20,high performance liquid chromatography（HPLC）sepa ration methods were used to conduct isolation of the petroleum ether fraction f rom the 95%EtOH extract of Commiphora myrrha.Finally,by using the meth od of spectrums,twenty-four compounds were isolated including fourteen sesqu iterpenes,eight triterpenes and two other classes.nine compounds were isolated from Commiphora for the first time,three compounds were isolated from Com miphora myrrha for the first time.To establish an HPLC method for the simultaneous determination of three sesquiterpenes from Commiphora myrrha,namely,rel-1S,2S-epoxy-4R-furan-oge rmacr-10（15）-en-6-one（compound I）,（1（10）E,2R,4R）-2-methoxy-8,12-epox ygermacra-1（10）,7,11-trien-6-one（compound II）and curzerenone[6,7-dihydr o-5β-isopropenyl-3,6β-dimethyl-6-vinylbenzofuran-4（5H）-one]（compound III）.Methods:The CAPCELL PAK C₁₈ column（250mm×4.6mm,5μm）was used wi th the mobile phase of acetonitrile（B）-0.1%phosphate（C）gradient elution,at the flow rate of 1.0mL.min^-1,the detection wavelength was 210nm,and the col umn temperature was at 30℃.Results:The linear ranges of compound I,com pound II and compound III were 0.0184⁰.4600μg（r=0.998）,0.0290⁰.7250μg（r=0.998）and 0.0258⁰.6450μg（r=0.999）,respectively;The precision（RSD<2%）and stability（RSD<2%）were good.The average recoveries（n=6）were 98.9%（RSD=0.48%）,98.6%（RSD=0.79%）,98.3%（RSD=0.60%）,respectively.The co ntent of compound I,compound II and compound III in six batches of sample s is 0.4483².0044,1.3991¹1.9190 and 1.0503³.9217mg.g^-1,respectively.