Relationship Between DNA Repair Gene XRCC1 And Susceptibility To Cervical Cancer

Objective:By studying the relationship between SNP locus of XRCC1 gene and cervical cancer,the genetic susceptibility of cervical cancer was determined,which provided a new idea for clinical diagnosis and prevention of cervical cancer.Methods: A comparative study was conducted to select 200 healthy persons,100 cases of cervical cancer and 80 cases,from April,2018 to March,2019 in Yijishan Hospital.Extraction of 5 ml venous anticoagulant and detection of(DNA)sequencing by in situ hybridization fluorescent staining of deoxynucleotides.The oligonucleotide chain of ddntp with complementary pair to be sequenced was labeled with 3’ end to recognize the sequence base.Ddntp oligonucleotide chains(labeled with fluorescent signals,but quenched)were labeled with 3’ ends,and the dna of leukocytes in the blood was stained with the oligonucleotide chain(Oligonucleotide),which was complementary to the base pairs to be sequenced.If the base is to be measured,then hybridization with it,send out fluorescence signal,through the instrument that can detect the fluorescence signal of the instrument,collect the signal,can report and analyze the karyotype of the target.One tube reaction can detect two bases,and can carry out multi-tube reaction,and then splice the result to realize chromosome karyotype analysis,and detect the distribution frequency of two snp loci of xrcc1 arg194 trp,arg 399 gln.The relationship between xrcc1 gene and cervical cancer risk,pathological type was analyzed.Results: In this study,5ml from venous blood of experimental group and control groupwas centrifuged to investigate the relationship between dna repair gene xrcc1 and cervical malignant tumor,xrccl gene arg194 trp single nucleotides polymorphism and cervical cancer.In terms of the risk of cervical intraepithelial neoplasia,chi-square test,p > 0.05,did not have statistical significance.There was no significant difference in the pathological type of cervical cancer,p > 0.05,so there was no difference in statistics.The relationship between arg399 gln single nucleotides polymorphism of xrcc L gene and the risk of cervical cancer and cervical intraepithelial neoplasia was carried out,and there was no significant difference between XRCCL gene single nucleotides polymorphism and the risk of cervical cancer and cervical intraepithelial neoplasia.Chi-square test showed that p < 0.05,which was statistically significant.The relationship between pathological types and pathological types was statistically significant(p < 0.05).Conclusion:The mutation of Arg194 Trp in XRCCl gene is not associated with the risk of cervical cancer,but the mutation of Arg399 Gln in XRCC1 gene increases the risk of cervical cancer;The Arg194 Trp polymorphism of XRCC l gene has no correlation with pathological type of cervical cancer,while the polymorphism of XRCC l gene Arg399G1 n may be related to the pathological type of cervical cancer and high expression in adenocarcinoma.