Study On The Metabolic Changes Of Hepcidin And GDF15 In Pregnant Women With Minor β-thalassemia Combined With IDA

ObjectiveThis study aimed at exploring the relationship,before and after oral iron supply treatment,between serum GDF15,Hepeidin,and iron metabolism-related indexes in minorβ-thalassemia combined with iron-deficiency anemia(IDA)pregnant women in different gestation,contributing to reveal this kind pregnant womens,iron metabolism traits,good to provide the scientific basis for the supply and monitor of iron supply treatment.MethodsFrom June 2017 to September 2018,we study 52 minor pregnant women with p-thalassemia combined with IDA(named’β+IDA group’),53 pregnant women with minor β-thalassemia(named’β group’),50 pregnant women with IDA(named ’IDA group’)and 59 healthy pregnant women(named’Healthy group’).We collected blood routine indexes and iron metabolism-related indexes and the content of serum GDF15 and Hepeidin were detected by ELISA kit.The relevant indexes of ’β+IDA group’and’IDA group’ were re-examined and obtained after taking oral iron supplements for 4 weeks.Statistical methods and spss20.0 software were used for comparison and correlation analysis of each index between and within the groups.Results1.As for’β+IDA group’ and’IDA group’,the levels of HGB,MCV,and iron metabolism-related indexes were significantly improved before and after iron supply treatrment,and the differences were statistically significant(P<0.05).The change trend of above indexes were consistent between the two groups.2.Pairwise comparison between the four groups showed no significant difference in GDF15 content and no statistical significance(P>0.05).There was no significant difference in GDF15 content between the before and after iron supply treatment in’β+IDA group’ and’IDA group’(P>0.05).There was no significant difference in Hepcidin content between ’β+IDA group’and’IDA group’(P>0.05).The Hepcidin level in ’β+IDA group’ and ’IDA group’ was significantly higher than that in’β group’and’Healthy group’,respectively,and the difference was statistically significant(P<0.05).In ’β+IDA group’ and ’IDA group’,Hepcidin content increased positively after iron supply treatment and the change was statistically significant(P<0.05).There was no statistical correlation between GDF15 and Hepcidin in all groups(P>0.05).3.There was a positive correlation between Hepcidin and ferritin in all groups,including before and after iron supply treatment,which was statistically significant.(In ’β+IDA group’:before treatment r＝0.376,p=0.006,aftertreatment r=0.471,p=0.019;In’IDA group’:before treatment r=0.674,p=0.000,after treatment r=0.762,p=0.000;In’β group’:r=0.578,p=0.000;In’p group’:r=0.633,p=0.000).4.The increase of ferritin after iron supply treatment in ’β+IDA group’is greater than that in’IDA group’,and the difference is statistically significant(P<0.05).Conclusion1.For pregnant women with minor β-thalassemia combined with IDA and those with IDA,oral iron supply therapy was effective.2.The pregnant women with minor β-thalassemia combined with IDA and the IDA had similar iron metabolism.Iron metabolism was negatively regulated by Hepcidin feedback,suggesting iron supply therapy was safe.3.There was difference in iron metabolism between the pregnant women with minor β-thalassemia combined with IDA and the IDA.The detection of ferritin and Hepcidin was helpful to reduce the risk of iron overload in iron supply therapy for the pregnant women withβ-thalassemia,which had clinical guiding significance.