Study On The Effect Of The Addition Of GSH To Tumescent Solution On Autologous Fat Graft Survival

Objective To investigate the effect of adding GSH to tumescent solution during fat graft harvest on autologous fat graft survival.Methods 14 male and female rabbits were divided into two groups randomly,7 in each group.With mimicking tumescent technique,the cervical and scapular region fat of the rabbits was extracted.Part of the fat granule is used for measuremennt of intracellular reactive oxygen species(ROS)content,and then partial fat is used for autologous particle fat transplantation in rabbits’ ears.Experimental group A:The donor areas of the rabbits were injected with 3 ml of tumescent solution with GSH.Control group B:The donor areas of the rabbits were injected with 3 ml regular tumescent solution.The grafts were harvested at 3 months after transplantation and assessed by general observation,volume measurement,wet weight measurement,HE staining for the number of fat cells,and CD34 immunohistochemical staining for the measurement of micro-vascular density.T test was performed by using SPSS 24.0.Results The intracellular ROS content of harvested fat cells in group A was lower than that in group B,and the difference was statistically significant(P<0.05).At 3months after transplantation,the wet weight of fat grafts in group A was(1133.21±87.97)mg and that in group B was(718.79±79.27)mg.The volume of fat grafts in group A was(1.00±0.04)ml and that in group B was(0.59±0.03)ml.The number of fat cells in group A was(746.6±15.7)个/10 high magnification vision and that in group B was(350.1±32.4)个/10 high magnification vision.The density in group A was(8.1±2.0)/high magnification vision and that in group B was(6.7±2.4)/highmagnification vision.The grafts’ volume,wet weight,and the number of fat cells in group A were higher than those in group B,and the differences were statistically significant(P<0.05).The difference of micro-vascular density between group A and group B had no statistical significance(P>0.05).Conclusion The addition of GSH to tumescent solution improves the preparation of tumescent solution,and it reduces oxidative stress in adipocytes,thereby improving the survival of fat graft.