The Changes Of Follicular Helper T Cells And Follicular Regulatory T Cells In Kawasaki Disease

Objective:To study the changes in expression of Tfh cells and Tfr cells in peripheral blood of acute Kawasaki disease in children.Provide some experimental basis for further study on the pathogenesis,diagnosis and treatment of Kawasaki disease.Methods:The Venous blood of children with Kawasaki disease(meets the diagnostic criteria for typical or atypical Kawasaki disease),general fever,and normal children in the pediatric department of the hospital from December 2016 to December 2018,20 patients in each of the 3 groups.Peripheral blood mononuclear cells in each specimen were extracted and added to LFTC CD4,LAPC CXCR5,LPerCP/Cy5.5 CD278(ICOS)and LPE FOXP3 for staining.Next,flow cytometry was used to analyze the expression levels of Tfh cells(CD4+CXCR5+ICOS+)and Tfr cells(CD4+Foxp3+CXCR5+ICOS+)in peripheral blood of these three groups and the change of Tfh/Tfr ratio.Finally,the data is statistically analyzed.Results 1.The proportion of Tfh cells in children with acute Kawasaki disease was significantly higher than that in general infection group and normal control group.The proportion of Tfr cells in children with acute Kawasaki disease was significantly lower than that in general infection group and normal control group,and Tfh/Tfr ratio in children with acute Kawasaki disease.Significantly higher than the general infection group and the normal control group(P<0.05).2.The proportion of Tfh cells,the proportion of Tfr cells and the ratio of Tfh/Tfr cells in children with acute fever were not significantly different from those in the normal control group.(P>0.05).Conclusion 1.Over-activation of Tfh and inhibition of Tfr expression may be involved in the immune pathogenesis of acute Kawasaki disease.2.There is no significant difference in the ratio of Tfh cells and Tfr cells between children with fever and normal children.I think that the difference between children with fever and normal children is not due to changes in the number of Tfh cells and Tfr cells,but may be due to changes in their function.