Expression Of OX1R In The Prefrontal Cortex And LHA Of Traumatic Brain Injury-induced Comatose Rats After LHA-DBS Stimulation

Objective: To investigate the wake-promoting effects of deep brain stimulation at lateral hypothalamic area(LHA-DBS) in comatose rats caused by Traumatic brain injury(TBI) and its mechanisms.Method: SD rats were randomly assigned to five different groups: control,sham-stimulated(TBI),stimulated(TBI+DBS),sham-antagonist and an antagonist(TBI+SB334867+DBS) group.The effects of DBS in a rat model of TBI-induced coma were observed and the level of conciousness were detected by EEG.Additionally,western blot,and immunofluorescence techniques were used to detect the levels of orexin 1 receptor(OX1R) expression in prefrontal cortex(PFC) and LHA tissue.Result: Results have shown that 11 rats were awakened in the control group,5 rats were awakened in the sham-stimulated group,10 rats were awakened in the stimulated group,8 rats were awakened in the sham-antagonist group and 11 rats were awakened in the antagonist group.Electroencephalogram(eeg) shows that wave accounted for the highest antagonist stimulating group,the hypothalamus stimulation group delta wave higher than the antagonist in the control group.WB results indicated that the concentration of OX1R in the different groups showed the following trend both in PFC and LHA: sham-stimulated group < antagonist group < stimulated group< sham-antagonist group< model group(P < 0.05).Immunofluorescence techniques results indicated that the concentration of OX1 R in the different groups showed the following trend both in PFC and LHA: sham-stimulated group < antagonist group < stimulated group< sham-antagonist group< model group(P < 0.05).Conclusion: Our findings suggest that LHA-DBS can promote consciousness recovery in rats which are comatose due to a traumatic brain injury.Additionally,upregulation of OX1R expression in the prefrontal cortex and LHA may be one mechanism involved in the consciousness-promoting effects of LHA-DBS.