1.effects On The Biological Behaviour Of Osteoblasts And Umbilical Vein Endothelial Cells By Biphasic Bioceramic Microspheres With Adjustable Biodegradation Rate 2.case Reports Of Personalized Systematic Treatment In Severe Chronic Periodontitis

Objective:To investigate the in vitro regulation of the proliferation and differentiation of primary mouse calvarial osteoblasts and human umbilical vein endothelial cells by porous core-shell ceramic microspheres with gradient composition characteristics and spatially dynamic degradation rate.Methods:Prepare precursors of three types of bilayered microspheres with 10%magnesium-doped calcium silicate(CSi-Mg10)and β-tricalcium phosphate(TCP):TCP/CSi-Mg10(mechanical mixture of TCP and CSi-Mg10),TCP@CSi-Mg10(with TCP in core layer and CSi-Mg 10 in shell layer),TCP@CSi-MglOp(pore-forming on shell layer of TCP@CSi-Mg10 with PS spheres((?)5μm)).Primary mouse calvarial osteoblasts were extracted by enzymatically digesting calvarial bone from neonatal mouse.The adhesion of osteoblasts on microspheres surface is observed by SEM.Then three groups of microspheres were extracted in osteoblast inductive conditional medium and applied to treat with primary osteoblasts in virous concentration.The proliferation of osteoblasts was evaluated by CCK-8 assay at Id,3d,5d.Osteogenic activity was evaluated by Alkaline phosphatase activity(ALP)test.Mineralization of osteoblasts was observed by Alizarin Red Staining.Prepared material extract with complete medium in various concentration to treat with human umbilical vein endothelial cells(HUVECs).The proliferation of HUVECs was evaluated by CCK-8 assay at 1d,3d,5d.Angiogenetic activity was evaluated by real-time quantitative PCR to test mRNA level of vascular endothelial growth factor(VEGF)and fibroblast growth factor(FGF).The migration of HUVECs was investigated by scratch test.Results:SEM showed all the surfaces of three types of microspheres in this study support the adhesion of osteoblasts via the extension of lamellipodia and filopodia,indicating its excellent biocompatibility.Additionally,the deposition of Hydroxyapatite layer could be observed on surfaces of all the groups.The material had no negative effects on proliferative activity of osteoblasts,even showing acceleration in short period(p<0.05).However,the high-concentration extraction(60 spheres/ml)significantly inhabited the proliferative activity of HUVECs(p<0.05).In ALP test and Alizarin Red Staining,two core-shell micospheres groups indicated higher ALP activity at day 14 in contrast to negative control,besides the TCP@CSi-Mg10p group showed peak OD value in Alizarin red semiquantitative analysis at day21.The migration of HUVECs treated with microsphere extraction was much faster than negative control(p<0.05).Furthermore,the migration in TCP@CSi-MglOp group presented outstanding speed than other groups at 24h(p<0.05).PCR showed the microspheres enhanced the expression of VEGF mRNA of HUVECs(p<0.05),and the expression in TCP@CSi-Mg10 and increased much significantly at 5 days(p<0.05).Conclusion:The biphasic microspheres constituted of TCP and CSi-Mg10 were of good biocompatibility,especially beneficial to the adhesion and early proliferation of osteoblasts.In comparison of Mechanical-mixed microspheres,the bilayer microspheres significantly accelerated the differentiation and mineralization of osteoblasts,as well as the VEGF expression in HUVECs.Besides,the enhanced porous connectivity structure on shell further promoted the osteogenesis of bilayer microspheres and its facilitation in HUVECs migration.This study was just a primary exploration of the core-shell structure and the porous connectivity structure,further experiments were necessary.Objective:Produce risk assessment of three patients with severe chronic periodontitis according to their personal risk factors,then develop a personalized periodontal systematic treatment and observe its clinical efficacyMethods:Three patients with severe chronic periodontitis were enrolled.After non-surgical periodontal treatment,to separately treat tooth with poor or hopeless prognosis,each patient’s risk factors were systematically considered and risk assessments were produced regularly.Afterward,long-term periodontal supportive treatment was adopted to control the inflammation.Results:After periodontal systematic treatment and follow-up,the tooth with poor prognosis were preserved and the full-mouth inflammation was generally controlled.Conclusion:Periodontal non-surgical treatment which is targeted to plaque control is valid for controlling full-mouth inflammation.Besides,systematically evaluating risk factors of each patients,such as the severity of periodontitis,destruction of periodontal supportive tissue,systematic factors and environmental factors,then precisely developing treatment for each patient or each site,would effectively improve the prognosis of individual teeth with Ⅲ degree mobility or Ⅱ/Ⅲ degree furcation.