MiR-512-5P Inhibits Human Glioma Cell Proliferation And Induces Cell Cycle Arrest By Directly Targeting JAG1

MicroRNAs(miRNAs)are small non-coding RNAs that inhibit gene expression during transcription and translation,and their dysregulation can lead to tumorigenesis.MiR-512-5p was found to be under expressed in glioblastoma,and thus,we examined the expression of miR-512-5p in glioma tissues using qRT-PCR and FISH.We then detected the effect of miR-512-5p overexpression in U87 and T98 cells using CCK-8,colony formation and EdU assays as well as flow cytometry.Jagged1(JAG1)was identified as a downstream target gene of miR-512-5p using a luciferase reporter gene and Western blottingting.The effect of JAG1 on glioma cells was then determined by Western blotting,CCK-8,colony formation,and EdU assays as well as by flow cytometry.In the functional recovery experiment,we found that overexpression of JAG1 reversed the effects of miR-512-5p.In vivo studies revealed that miR-512-5p inhibited tumour growth,prolonged the overall survival of nude mice and reduced the protein levels of JAG1,CD31 and Ki67.We concluded that miR-512-5p is a new target for the molecular therapy of glioma.MiR-512-5p inhibits the proliferation of glioma by first inhibiting JAG1,also induces cell cycle arrest and thus plays a major role as a tumour suppressor gene.