The Effects Of PES On Autophagy And Drug Resistance Of SGC-7901 In Gastric Cancer Under The Condition Of Hyperthermia

objective:To investigate the effects of heat shock protein 70(HSP70)inhibitor PES on autophagy and drug resistance of SGC-7901 in gastric cancer.Method:Divide the logarithmic growth cells of gastric cancer SGC-7901 into four groups.:A group:43℃ DMSO group,B group:37℃DMSO group,C group:43℃ PES group,D group:37℃ PES group The cells were cultured for 6 hours under the set conditions,Four groups of cells were cultured,The expression of heat shock protein 70,p-glycoprotein(p-pg)and autophagic marker protein(Beclin1)were observed in each group by Western blot.Result:The expression of HSP70 in each group was: A group(72.92±0.05)、B group DMSO(62.93±0.04)、C group(40.91±0.04)、D group(27.97±0.02).The result showed that the gastric cancer SGC-7901 cells in each group could express HSP70.Group A VS Group B(72.92±0.05 VS 62.93±0.04),shows that heat treatment(43℃)under the condition of SGC7901 gastric cancer cells can be induced by high expression of HSP70,make the HSP70 expression level increased(P <0.05);Group A VS Group C(72.92±0.05 VS 40.91±0.04)and group B VS group D(62.93±0.04 VS 27.97±0.02),showed that under the condition of37℃ and 43℃ PES can inhibit the expression of HSP70 of SGC7901 gastric cancer cells,lower the standards of HSP70 expression(P < 0.05).The expression of P-gp in each group was: A group(59.52±0.02)、B group DMSO(53.95±0.04)、C group(56.73±0.03)、 D group(41.31±0.04).The result showed that the gastric cancer SGC-7901 cells in each group could express P-gp.Group A VS Group B(59.52±0.02 VS53.95±0.04),shows that heat treatment(43 ℃)under the condition of SGC7901 gastric cancer cells can be induced by high expression of P-gp,make the P-gp expression level increased(P < 0.05);Group A VS Group C(59.52±0.02 VS 56.73±0.03)and group B VS group D(53.95±0.04 VS41.31±0.04),showed that under the condition of 37℃ and 43℃ PES can inhibit the expression of P-gp of SGC7901 gastric cancer cells,lower the standards of P-gp expression(P < 0.05).The expression of Beclin1 in each group was: A group(88.09±0.05)、B group DMSO(68.79±0.03)、C group(56.73±0.03)、D group(41.31 ± 0.04).The result showed that the gastric cancer SGC-7901 cells in each group could express Beclin1.Group A VS Group B(88.09±0.05 VS 68.79±0.03),shows that heat treatment(43℃)under the condition of SGC7901 gastric cancer cells can be induced by high expression of Beclin1,make the Beclin1 expression level increased(P <0.05);Group A VS Group C(88.09±0.05 VS 56.73±0.03)and group B VS group D(68.79±0.03 VS 41.31±0.04),showed that under the condition of 37 ℃ and 43 ℃ PES can inhibit the expression of Beclin1 of SGC7901 gastric cancer cells,lower the standards of Beclin1 expression(P < 0.05).Conclusion:PES could inhibit the expression of P-gp protein and Beclin1 protein under hyperthermia.The mechanism may be related to the inhibition of PES-induced HSP70 expression in gastric cancer SGC-7901 cells.PES can affect autophagy and drug resistance in gastric cancer SGC-7901 cells under hyperthermia conditions.