Effect Of TERT Promoter Mutation On The Polarization And Function Of Microglia/Tumour-Associated Macrophages In Primary Glioblastoma

Objective:To explore the influence of TERT promoter mutation on the polarization and function of microglia/tumor-associated macrophages in primary glioblastoma by detecting the expression of M1 and M2 microglia/tumor-associated macrophages in TERT promoter mutant and wild type primary glioblastoma.Methods:Seventy-nine tumor paraffin sections of patients who underwent surgical treatment and pathologically diagnosed as primary glioblastomain in our hospital from 2015 to2018 were selected.Forty-three specimens were divided into TERT promoter mutation group and thirty-six into wild-type group according to the presence of TERT promoter mutation.Inducible nitric oxide synthase(iNOS)was used as the marker of M1-type cells,and arginine enzyme 1(Arg-1)was used as the marker of M2-type cells.The immunohistochemistry method was used to detect the number of positive cells of iNOS and Arg-1 in the two groups.Results:In primary glioblastoma,M1-type microglia/tumor-associated macrophages are more than M2-type ones.M1-type microglia/tumor-associated macrophages showed no significantdifferencebetweenTERTpromotermutantgroupandwild group(p=0.08).M2-type microglia/tumor-associated macrophages were more expressed in TERT promoter mutant group than in wild group,and the difference was statistically significant(P<0.05);In addition,in TERT promoter mutant group,the vessels with positive M2-type cells are more than that in TERT promoter wild group,and the difference was statistically significant(P<0.05).Conclusion:M1-type TAMs plays a major role in the occurance of primary glioblastoma,while M2-type TAMs plays an important role in the progression of primary glioblastoma.The TERT promoter mutation induced the polarization of TAMs to M2-type and enhanced the M2-type cells~'s functional activity such as angiogenesis.