The Research Of The Pesticide Effect And Mechanism Of Prunella Vulgaris On Rats With Autoimmune Thyroiditis

Objective:Heterologous thyroid antigen immunization was coordinated with high iodine to make an AIT rat model.The influences of Prunella vulgaris on thyroid histomorphology,autoantibody and thyroid hormones,and relevant cytokines of AIT rats were observed.Meanwhile,the pesticide effect of using Prunella vulgaris to cure AIT and its immunomodulatory effect mechanism were discussed to provide the experimental basis for clinical application.Methods:40 SPF Lewis female rats were divided into two groups according to the random number table,10 in the normal group and 30 in the experimental group.The molding method for the experimental group included porcine thyroglobulin with Freund’s incomplete adjuvant and complete adjuvant to do multi-point subcutaneous injection.After validation,the experimental group was randomly divided into model group,tripterygium glycoside group,and Prunella vulgaris group.Each group had 10rats.Verified by molding,rats in tripterygium glycosides group（6.3mg/kg）and Prunella vulgaris group（2.7g/kg）were conducted gavage administration in accordance with 1ml/100g.d dosage.All rats were narcotized through the enterocoelia.After operating in ice bags,thyroid was taken from the neck of living body.Through the HE staining method,histomorphology was observed under the light microscope.Blood was taken from aorta abdominalis.After centrifuge,supernatant was extracted.ELISA method was used to detect TPOAb,TGAb,TSH,FT₃,FT₄,T₃,T₄ and Th1 cytokines IL-1α,TNF-α,IFY-γand Th2 cytokines IL-4,IL-6 and IL-10.SPSS24.0 software was used to sort out data and summarize discussion.Results:1.Histomorphology:under the light microscope,thyroid tissue of the model group showed lots of damaged thyroid crypt.There was the extensive lymphocytes infiltration in intercellular substance.After using tripterygium glycosides and Prunella vulgaris for intervention,morphology of thyroid tissue was obviously alleviated by comparing with the model group.Some crypt structure was slightly destroyed,showing lymphocytes infiltration in mesenchyme.2.Thyroid autoantibody and thyroid hormones:comparing with the normal group,TPOAb,TGAb,TSH,FT₃,FT₄,T₃,T₄ of rats in the model group were significantly increased.There was the statistical significance（P<0.05）.By comparing with the model group,tripterygium glycosides and Prunella vulgaris group were significantly reduced.There was the statistical significance（P<0.05）.By making a comparison between Prunella vulgaris group and tripterygium glycosides group,TPOAb and TGAb in Prunella vulgaris group were reduced to some extent.There was the statistical difference（P<0.05）.3.Cytokines:（1）Th1 cytokines（IL-1α,TNF-αand IFY-γ）results:by comparing with the normal group,IL-1α,TNF-αand IFY-γof rats in the model group were significantly increased.There was the statistical significance（P<0.01）.By comparing with the model group,cytokines in tripterygium glycosides group and Prunella vulgaris group were significantly reduced.There was the statistical difference（P<0.05）.Comparing with the tripterygium glycosides group,TNF-αin Prunella vulgaris group was reduced to some extent.There was the statistical difference（P<0.05）.（2）Th2cytokines（IL-4,IL-6 and IL-10）results:comparing with the normal group,IL-4,IL-6and IL-10 of rats were significantly increased（P<0.01）.Comparing with the model group,IL-4,IL-6 and IL-10 in tripterygium glycosides group and Prunella vulgaris group were reduced to some extent（P<0.05）.Both of them had no obvious difference.Conclusions:1.Prunella vulgaris can alleviate and improve lymphocytes infiltration of AIT rats’thyroid tissue mesenchyme.2.Prunella vulgaris can reduce thyroid autoantibody TPOAb and TGAb,also can reduce thyroid hormones.Moreover,comparing with tripterygium glycosides,Prunella vulgaris shows some advantages to reduce TPOAb.Prunella vulgaris can improve thyroid function and showing some therapeutic action on AIT.3.Prunella vulgaris can reduce Th1 and Th2 cytokines.By restraining excessive secretion of cytokines and down-regulate concentration of cytokines,so as to alleviate damage of thyroid tissue,improve pathological state of AIT rats,alleviate and delay AIT,showing some therapeutic action on AIT.