| PurposeLung cancer is the leading cause of cancer-related death worldwide.Immune response is essential for the development of lung cancer,and presence of tumor-infiltrating lymphocytes in the tumor microenvironment is considered to be an indication of the host immune response to tumor antigens.The aim of this study was to examine the expression of CD45RO,CD8,CCR7,Foxp3 and their correlation with the clinicopathological features and survival of patients with lung adenocarcinoma..Patients and methodsNinety patients with lung adenocarcinoma who underwent surgery were recruited in the present study.Lung adenocarcinoma tissues and paired adjacent lungtissues were obtained from all participants.Immunohistochemistry was performed to detect expression of CD45RO,CD8,CCR7and Foxp3.After multiplying thestaining intensity score by the labeling frequency score,the immunohistochemical results were divided into three groups:TILs Low,TILs Intermediate and TILs High.ResultsCD45RO~+,CD8~+and CCR7~+infiltrating lymphocytes was significantly increased in lung adenocarcinoma(all p<0.001),whereas Foxp3~+infiltrating lymphocytes was decreased(p<0.001)compared with than in adjacent tissues.CD45RO~+TILs were negatively associated with tumor size(p=0.002),lymph node metastasis(p<0.001)and TNM stage(p<0.001).CD8~+TILs were also negatively correlated with lymph node metastasis(p=0.016).Kaplan–Meier curve analysis revealed that CD45RO~+TILs were positively associated with longer disease-free survival(DFS)(p<0.001)and overall survival(OS)(p<0.001).Univariate and multivariate Cox’s proportional hazards regression confirmed that CD45RO~+TILs(high)independently predicted longer DFS(p=0.002)and OS(p=0.009).ConclusionThe present study demonstrates that CD45RO~+TILs are negatively correlated with tumor size,lymph node metastasis and TNM stage and that CD45RO~+TILs(High)can be regarded as a novel and promising biomarker for prolonged DFS and OS in lung adenocarcinoma patients. |
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