Expression And Clinical Significances Of RANKL And MMP-2 In External Auditory Canal Cholesteatoma

ObjectivesTo detect the differential expression of RANKL and MMP-2 in external auditory canal cholesteatoma and external ear canal skin,and to explore the significance of their expression in external auditory canal cholesteatoma disease and the regulation mechanism in the process of bone destruction.It provides a theoretical basis for the prevention and treatment of external auditory canal cholesteatoma.MethodsThe expression of RANKL and MMP-2 in 47 cases of external auditory canal cholesteatoma and 18 cases of normal external auditory canal tissue were detected by immunohistochemistry.Results1 The positive expression rates of RANKL and MMP-2 in external auditory canal cholesteatoma were 76.60%and 78.72%,and the positive expression rates in normal external auditory canal tissue were 11.11%and 16.67%.RANKL and MMP-2 were in external auditory canal cholesteatoma.The positive expression rate in the tissue was significantly higher than that in the normal external auditory canal tissue,and the difference between the two groups was statistically significant(χ~2=22.983、21.177,P<0.05).2 In external auditory canal cholesteatoma,the expression of RANKL and MMP-2were significantly associated with the the degree of bone destruction(P﹤0.05).3 The positive expression of RANKL and MMP-2 were 76.60%and78.72%,respectively,in which co-positive were 33 cases and co-negative were 7 cases.The RANKL was correlated with the MMP-2 expression(r=0.572,P=0.000).Conclusions1 The expression levels of RANKL and MMP-2 are related to the cholesteatoma of the external auditory canal.The positive expression rates of RANKL and MMP-2 in the cholesteatoma of the external auditory canal are significantly increased.2The expression levels of RANKL and MMP-2 are related to the degree of bone destruction in external auditory canal cholesteatoma,and the higher the positive expression rates of both,the more serious the bone destruction in external auditory canal cholesteatoma.3There was a positive correlation between the expression of RANKL and MMP-2 in external auditory canal cholesteatoma.Figure 4,Table 9,Reference 40...