Study On Quality Characterization Of Banxia Xiexin Decoction Standard Decoction

Chinese traditional medicine has formed a precious culture of traditional chinese medicine since ancient times.In order to better inherit and develop the culture of traditional chinese medicine and serve patients,relevant institutions of chinese traditional medicine have integrated some prescriptions used by modern chinese medicine doctors according to patients'symptoms.These prescriptions have the characteristics of exact curative effect and high safety through large clinical data analysis.They have been summarized into classical prescriptions and classical names.Formula is the crystallization of chinese medicine for thousands of years.It is very important for patients and the development of chinese medicine to give full play to the greatest value of classical prescriptions and to make good medicines.This topic selected Banxia Xiexin Decoction which has good clinical therapeutic effect on gastrointestinal diseases from the first batch of classical research catalogues published by the relevant institutions of chinese medicine as the research object,and carried out relevant research on the characterization of the material benchmark quality of Banxia Xiexin Decoction for later stage.Researchers have laid a foundation for the development and research of Banxia Xiexin Decoction.Material benchmark refers to the reference of standardized compound preparations prepared according to the dosage and decocting method recorded in the original ancient books.Except for the different moulding techniques,the other preparation processes should be basically consistent with the records in the ancient books.The establishment of quality standards for reference substances provides a new research direction for the consistency and stability of modern chinese medicine dosage forms.This subject takes the material standard of Banxia Xiexin Decoction as the research object,and studies its quality characterization methods from identification,multi-component content determination,fingerprint and so on.The main research contents are as follows:?1?Banxia Xiexin Decoction is composed of pinellia ternata 9g,dried ginger 6g,scutellaria baicalensis 6g,coptis chinensis 3g,ginseng 6g,roasted licorice 6g and jujube 12g.According to the relevant requirements of the ministry of health and the state administration of traditional chinese medicine,the preparation process of Banxia Xiexin Decoction is further decocted and concentrated into freeze-dried powder,which is the material standard of Banxia Xiexin Decoction.?2?A method for the determination of 6-gingerol in dried ginger by high performance liquid chromatography was established.The method was negative and had no interference.The results of the methodological investigation were in accordance with the relevant provisions of the chinese pharmacopoeia.?3?A method for the determination of baicalin and berberine hydrochloride in scutellaria baicalensis and coptis chinensis under the same chromatographic conditions was established by high performance liquid chromatography.The results of the methodological investigation were in conformity with the relevant provisions of the chinese pharmacopoeia pharmacopoeia.?4?A method for the determination of ginsenoside Rb₁ in panax ginseng by high performance liquid chromatography was established.The results of the methodological investigation were in conformity with the relevant provisions of the chinese pharmacopoeia.?5?A method for the determination of glycyrrhizin and glycyrrhizic acid in roasted licorice by high performance liquid chromatography was established.The results of the methodological investigation were in conformity with the relevant provisions of the chinese pharmacopoeia.?6?To establish the fingerprint of the medium polar part of Banxia Xiexin Decoction,and determine four characteristic peaks in the fingerprint of the medium polar part of Banxia Xiexin Decoction,namely baicalin,ginsenoside Rb₁,glycyrrhizic acid and berberine hydrochloride.At the same time,comparing the retention time of the peaks of Banxia Xiexin Decoction with those of the single herbs and negative ones,25peaks were identified,including 15 peaks from Scutellaria baicalensis,6 peaks from coptis chinensis,2 peaks from roasted licorice,1 peak from dried ginger and 1 peak from ginseng.?7?TLC identification of dried ginger and ginseng in the reference material,negative without interference.The results of the methodological investigation were in conformity with the relevant provisions of the chinese pharmacopoeia.