Correlation Study Of Estrogen,Peripheral Blood Immune Cells,ALP And GGT In PBC Patients

Objective:The changes in the levels of sex hormones and immune cells in the peripheral blood of patients with primary biliary cholangitis(PBC)were clarified,so as to improve the understanding of PBC and find new treatment methods.Methods:Patients diagnosed with PBC in the second hospital of shanxi medical university from March 1,2013 to December 1,2018 were selected as the experimental group and healthy people as the control group.Fasting venous blood was extracted from all enrolled people.ALP levels were measured by NPP substrate-AMP buffer method,GGT level was measured by rate method,estradiol and progesterone level by chemiluminescence method,and Th2 and Th17 cell levels were measured by flow cytometry.The differences of ALP,GGT,estrogen,progesterone,Th2 cells and Th17 cells between the two groups were compared.Results:1.1.There was no significant difference in age and gender between PBC group and normal group.The average age of PBC patients was 57.93 years old,and the proportion of male and female patients was 18.5% and 81.5% respectively.2.Compared with the normal control group,estrogen and progesterone levels in the PBC group were significantly higher than those in the normal control group(P<0.05,the differences were statistically significant),and Th2 cell levels weresignificantly lower than those in the normal control group(P<0.05,the differences were statistically significant),and the level of Th17 cells increased,but P>0.05 showed no statistical significance.3.The results of the study on the correlation among liver biochemical indexes,estrogen and immune cells in the PBC group showed that the changes in ALP and GGT levels were not correlated with the changes in estrogen,progesterone,Th2 cells and Th17 cells,and the changes in estrogen and progesterone levels were not significantly correlated with the changes in Th2 cells and Th17 cells.Conclusion:1.The number of women with PBC was significantly higher than that of men.2.Cholestasis is not associated with estrogen in paitients with primary biliary cholangitis.3.The increase of ALP and GGT may be related to the decrease of immune suppression caused by Th2.4.The increase trend of Th17 cells in PBC group was not statistically significant compared with the control group,which may be related to the small number of cases.